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Updated: Mar 21, 2026

Microfluidic Chip Fabrication and Method to Detect Influenza

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

Integrated sample-to-detection chip for nucleic acid test assays.

R Prakash¹, K Pabbaraju¹, S Wong¹

¹Provincial Laboratory for Public Health of Alberta, Calgary, 3030 Hospital Drive NW, Calgary, AB, T2N 4W4, Canada.

Biomedical Microdevices

|May 12, 2016

Summary

This study presents an integrated microfluidic chip for nucleic acid extraction and real-time PCR. The novel lab-on-chip device enables sensitive and specific infectious agent detection with reduced sample volumes.

Keywords:

Dielectrophoresis Droplet microfluidics Electrowetting Lab-on-a-chip Nucleic acid tests Sample-to-detection

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Area of Science:

Biotechnology
Microfluidics
Molecular Diagnostics

Background:

Nucleic acid amplification tests (NATs) are crucial for infectious agent detection.
Current NATs often require separate platforms for nucleic acid extraction and real-time PCR.
Microfluidic lab-on-chip (LOC) technologies offer potential for integrated diagnostic solutions.

Purpose of the Study:

To develop and validate an integrated microfluidic LOC device for simultaneous nucleic acid extraction and real-time, reverse transcription (RT) PCR (qRT-PCR).
To assess the performance of the integrated NAT chip compared to standard diagnostic platforms.

Main Methods:

Implementation of an electro-actuation based LOC micro-device for sample and reagent droplet manipulation.
Integration of chemical lysis with electric field-assisted nucleic acid isolation in a parallel processing scheme.
Incorporation of a four-channel parallel qRT-PCR amplification and detection assay on the same chip, utilizing dielectrophoresis, electrostatic/electrowetting actuation, micro-heaters, and temperature sensors.

Main Results:

The integrated NAT chip successfully performed chip-based nucleic acid extraction and qRT-PCR from clinical samples.
Validation using clinical samples demonstrated comparable sensitivity and specificity to standard platforms.
The system achieved up to a 10-fold reduction in sample and reagent volumes.

Conclusions:

The developed integrated NAT chip offers a promising solution for point-of-care molecular diagnostics.
This microfluidic system streamlines NATs by combining extraction and amplification on a single device.
The technology enables efficient and sensitive detection of infectious agents with reduced resource requirements.