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Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
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Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
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Updated: Mar 21, 2026

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
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Transient Expression Systems in Plants: Potentialities and Constraints.

Tomas Canto1

  • 1Centro de Investigaciones Biológicas (CIB, CSIC), Ramiro de Maeztu 9, 28040, Madrid, Spain. tomas.canto@cib.csic.es.

Advances in Experimental Medicine and Biology
|May 12, 2016
PubMed
Summary

Transient expression in plants offers a faster alternative to stable transformations for producing valuable gene products. This method allows efficient gene overexpression for research and commercial use, bypassing complex plant genetic engineering.

Keywords:
Transient expression by agroinfiltrationTransient expression from plant virus vectorsTransient expression in plants by biolistic bombardmentTransient expression systems in plants

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Area of Science:

  • Plant biotechnology
  • Molecular biology
  • Biotechnology

Background:

  • Plants have historically been utilized for extracting valuable compounds.
  • Emerging technologies enable plants to serve as efficient transient expression systems for specific genes.
  • Transient expression offers an alternative to stable plant transformation, avoiding regulatory hurdles.

Purpose of the Study:

  • To describe strategies for transient gene expression in plants.
  • To discuss biological challenges and solutions for transient expression.
  • To compare the advantages and limitations of different expression methods.

Main Methods:

  • Overview of current transient gene expression strategies in plants.
  • Analysis of plant defense mechanisms against foreign genetic material.
  • Evaluation of methods to overcome expression limitations.

Main Results:

  • Transient expression systems provide a rapid and efficient means to produce gene products.
  • Understanding and mitigating plant resistance responses are crucial for successful expression.
  • Each strategy presents unique benefits and drawbacks that must be considered.

Conclusions:

  • Transient gene expression in plants is a powerful tool for rapid production of target proteins.
  • Addressing biological barriers enhances the efficiency of plant-based expression systems.
  • Informed selection of expression strategies maximizes success in plant molecular farming.