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Published on: February 4, 2015
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CD146(+) cells are essential for kidney vasculature development.
Kimmo J Halt1, Heikki E Pärssinen1, Sanna M Junttila1
1Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland; Biocenter Oulu, Oulu, Finland; Center of Excellence in Cell-Extracellular Matrix Research, Oulu, Finland.
Kidney International
|May 12, 2016
Summary
Kidney vasculature development relies on specific endothelial cells (ECs). CD146(+) cells are crucial for regenerating the EC network in embryonic kidneys, highlighting their role in renal vascular assembly.
Area of Science:
- Developmental biology
- Renal physiology
- Vascular biology
Background:
- Kidney vasculature is vital for renal function.
- Mechanisms of kidney vascular development are poorly understood.
- Limited models exist for studying kidney vascular assembly dynamics.
Purpose of the Study:
- Investigate endothelial cell (EC) heterogeneity in embryonic kidneys.
- Characterize the dynamics of kidney vasculature development.
- Identify critical cell populations for renal vascular assembly.
Main Methods:
- Utilized Tie1Cre;R26R(YFP)-based fate mapping.
- Employed time-lapse embryonic kidney organ culture.
- Developed novel dissociation and reaggregation technology for cell depletion and fate mapping.
Main Results:
- Demonstrated EC heterogeneity based on VEGFR2, CD31, and CD146 markers.
- Depletion of Tie1(+) or CD31(+) ECs showed substantial network regeneration.
- Depletion of CD146(+) cells abolished EC regeneration, indicating their critical role.
- Fate mapping confirmed CD146(+)/CD31(-) cells differentiate into CD31(+) ECs.
Conclusions:
- Ex vivo embryonic kidney culture models offer novel approaches for studying renal EC development.
- CD146(+) cells are essential for kidney vasculature development and regeneration.
- VEGF signaling is critical, with VEGF and erythropoietin expressed constitutively in the embryonic kidney.
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