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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR01:59

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Transgenic Plants02:50

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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
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Plant Breeding and Biotechnology01:59

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Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
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Related Experiment Video

Updated: Mar 21, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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Targeted Gene Manipulation in Plants Using the CRISPR/Cas Technology.

Dandan Zhang1, Zhenxiang Li1, Jian-Feng Li1

  • 1State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.

Journal of Genetics and Genomics = Yi Chuan Xue Bao
|May 12, 2016
PubMed
Summary

CRISPR/Cas technology offers versatile plant gene manipulation beyond editing. This review covers recent plant applications, performance improvements, and strategies to minimize off-target effects, drawing insights from mammalian research.

Keywords:
CRISPR/Cas technologyGene knockoutGenome editingPlants

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Area of Science:

  • Plant science
  • Molecular biology
  • Biotechnology

Background:

  • CRISPR/Cas technology is a powerful genome editing tool with broad applications.
  • Its utility extends beyond editing to various sequence-specific gene manipulations.

Purpose of the Study:

  • To review recent CRISPR/Cas applications in plants.
  • To discuss methods for enhancing CRISPR/Cas performance.
  • To explore strategies for reducing off-target effects in plant gene editing.

Main Methods:

  • Literature review of recent CRISPR/Cas applications in plants.
  • Analysis of factors influencing CRISPR/Cas efficiency and specificity.
  • Incorporation of advancements from mammalian CRISPR/Cas research.

Main Results:

  • CRISPR/Cas has evolved into a versatile toolkit for plant gene manipulation.
  • Key factors for improving performance and reducing off-target effects have been identified.
  • Insights from mammalian research offer potential for plant applications.

Conclusions:

  • The CRISPR/Cas toolkit holds significant potential for plant research.
  • Further exploration and application of CRISPR/Cas technologies in plants are encouraged.
  • Optimizing performance and specificity are crucial for successful plant genome engineering.