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Related Concept Videos

Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

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Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
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Atomic Emission Spectroscopy: Interference01:30

Atomic Emission Spectroscopy: Interference

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In atomic emission spectroscopy (AES), high-temperature atomizers excite a broad range of elements and molecules that generate complex emissions from sources such as oxides, hydroxides, and flame combustion products in the flame or plasma. Several strategies can be employed to minimize spectral interferences caused by overlapping emission lines or bands. These include increasing instrument resolution, choosing alternative emission lines, optimally placing the detector in low-background regions,...
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EDTA: Direct, Back-, and Displacement Titration01:30

EDTA: Direct, Back-, and Displacement Titration

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The EDTA titration types for metal ion analysis include direct titration, back-titration, and replacement titration.
Direct titration involves buffering the metal ion solution to the desired pH and directly titrating with standard EDTA until the endpoint. The optimum pH ensures a large conditional formation constant of metal−EDTA and visibility of the free indicator color in the solution. In addition, auxiliary complexing reagents are used to prevent the precipitation of metal hydroxides...
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EDTA: Indirect and Alkalimetric Titration01:23

EDTA: Indirect and Alkalimetric Titration

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Unlike direct titration, back-titration, and displacement titration, indirect titration is an EDTA titration method for quantifying anions. In the indirect titration method, anions are precipitated as their insoluble salts with excess metal ions. The filtrate containing the excess metal ions is directly titrated with standard EDTA until the endpoint is achieved. Another approach involves extracting the metal ion and back-titrating with standard EDTA to obtain the endpoint. In this way, the...
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Atomic Absorption Spectroscopy: Interference01:25

Atomic Absorption Spectroscopy: Interference

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Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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Electrochemiluminescence Assays for Human Islet Autoantibodies
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EDTA interference in electrochemiluminescence ACTH assay.

Burak Toprak1, Hulya Yalcin2, Elif Arı2

  • 11 Department of Clinical Biochemistry, Silopi State hospital, Silopi, Şırnak, Turkey.

Annals of Clinical Biochemistry
|May 12, 2016
PubMed
Summary

High EDTA concentration in plasma significantly lowers Roche's adrenocorticotropic hormone (ACTH) assay results. Inadequately filled EDTA tubes can cause substantial ACTH measurement errors, necessitating specimen rejection.

Keywords:
ACTHEDTAplasma

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Area of Science:

  • Clinical Chemistry
  • Endocrinology
  • Laboratory Medicine

Background:

  • Plasma is the recommended sample type for the Roche adrenocorticotropic hormone (ACTH) assay.
  • The impact of varying ethylenediaminetetraacetic acid (EDTA) concentrations on the Cobas ACTH assay requires investigation.

Purpose of the Study:

  • To evaluate the effect of increased EDTA concentration on the accuracy of the Cobas ACTH assay.
  • To determine the clinical significance of EDTA concentration in plasma samples for ACTH measurement.

Main Methods:

  • Plasma samples were prepared with twofold and fourfold higher EDTA concentrations.
  • Under-filled K2EDTA tubes (50% and 25% filled) were used to create variable EDTA concentrations.
  • ACTH concentrations were measured using the Cobas ACTH assay with four replicates for each condition.

Main Results:

  • Elevated EDTA concentrations led to a significant reduction in measured ACTH levels.
  • A 50% filled EDTA tube resulted in a 19% decrease in ACTH concentration.
  • A 25% filled EDTA tube showed a 50% decrease in ACTH concentration.

Conclusions:

  • Inadequately filled EDTA specimens can compromise the accuracy of the Cobas ACTH assay.
  • It is recommended to reject EDTA specimens with insufficient fill volume when performing the Cobas ACTH assay.
  • Proper sample collection and handling are crucial for reliable ACTH testing.