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Updated: Mar 21, 2026

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink
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Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink.

Aleksander Skardal1, Mahesh Devarasetty2, Hyun-Wook Kang2

  • 1Wake Forest Institute for Regenerative Medicine, Wake Forest Univeristy Health Sciences; askardal@wakehealth.edu.

Journal of Visualized Experiments : Jove
|May 12, 2016
PubMed
Summary

Researchers developed a novel hyaluronic acid and gelatin-based bioink for bioprinting. This advanced bioink supports cell viability and function, enabling the creation of functional tissue constructs for drug screening and potential organ replacement.

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Area of Science:

  • Biotechnology
  • Materials Science
  • Regenerative Medicine

Background:

  • Bioprinting technology offers potential for creating tissue-engineered organs for transplantation and organoids for drug screening.
  • Current bioprinting applications are limited by the availability of suitable bioinks that support cell viability and provide tissue-specific cues.
  • Hyaluronic acid (HA) and gelatin are biocompatible polymers with potential for developing advanced bioinks.

Purpose of the Study:

  • To develop a versatile hyaluronic acid (HA) and gelatin-based hydrogel system for bioprinting.
  • To create bioinks that can mimic in vivo tissue mechanical properties and provide tissue-specific biochemical signals.
  • To demonstrate the utility of the developed bioink system for creating functional in vitro liver constructs.

Main Methods:

  • A multi-crosslinker, 2-stage crosslinking protocol was employed using hyaluronic acid and gelatin.
  • Tissue-derived extracellular matrix materials and growth factors were incorporated to provide biochemical cues.
  • Polyethylene glycol (PEG)-based crosslinkers with varying properties were used to control construct mechanical properties.
  • Primary liver spheroids were bioprinted using the developed bioink to create liver constructs.

Main Results:

  • The developed hydrogel system allowed for tunable mechanical properties ranging from 100 Pa to 20 kPa.
  • Bioprinted liver constructs exhibited high cell viability.
  • The constructs demonstrated measurable functional output, including albumin and urea production.
  • The bioink system proved versatile for creating tissue-specific constructs.

Conclusions:

  • A versatile HA and gelatin-based hydrogel bioink system was successfully developed.
  • This system enables the creation of bioprinted constructs with tunable mechanical properties and biochemical cues.
  • The developed methodology provides a framework for fabricating various tissue-engineered constructs for regenerative medicine and drug discovery.