Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Human placental trophoblasts support sustained <i>Treponema pallidum</i> replication and reveal new candidate host pathways implicated in congenital syphilis.

bioRxiv : the preprint server for biology·2026

Same author

Li-Fraumeni Syndrome-Associated p53 Variants Disrupt Kidney and Urinary Tract Development.

medRxiv : the preprint server for health sciences·2025

Same author

The dynamics of tubulogenesis in development and disease.

Development (Cambridge, England)·2025

Same author

Comparative analysis of <i>Xenopus</i> mesonephric transcriptomics: Conservation of the developmental lineage of nephron stages.

bioRxiv : the preprint server for biology·2025

Same author

Implementation of a geriatric assessment SmartPhrase: A multi-institutional pilot study.

Journal of the American Geriatrics Society·2024

Same author

Using the Geriatric 5Ms to teach structural and social determinants of health.

Journal of the American Geriatrics Society·2023

Search research articles

Related Experiment Video

Updated: Mar 21, 2026

Technique to Target Microinjection to the Developing Xenopus Kidney

Technique to Target Microinjection to the Developing Xenopus Kidney

Published on: May 3, 2016

Technique to Target Microinjection to the Developing Xenopus Kidney.

Bridget D DeLay¹, Vanja Krneta-Stankic², Rachel K Miller³

¹Department of Pediatrics, Pediatric Research Center, University of Texas McGovern Medical School.

Journal of Visualized Experiments : Jove

|May 12, 2016

Summary

This study details a method for targeting the developing frog kidney (pronephros) using microinjection in Xenopus embryos. This technique allows precise gene manipulation for studying kidney development and disease models.

More Related Videos

Microinjection of Xenopus Laevis Oocytes

Microinjection of Xenopus Laevis Oocytes

Published on: February 23, 2009

Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles

Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles

Published on: September 4, 2019

Related Experiment Videos

Last Updated: Mar 21, 2026

Technique to Target Microinjection to the Developing Xenopus Kidney

Technique to Target Microinjection to the Developing Xenopus Kidney

Published on: May 3, 2016

Microinjection of Xenopus Laevis Oocytes

Microinjection of Xenopus Laevis Oocytes

Published on: February 23, 2009

Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles

Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles

Published on: September 4, 2019

Area of Science:

Developmental biology
Comparative physiology
Genetics

Background:

The embryonic kidney of Xenopus laevis, the pronephros, serves as a valuable model for kidney disease research.
Xenopus embryos offer advantages for experimental manipulation due to their size, external development, and established fate maps.

Purpose of the Study:

To describe a protocol for targeted microinjection into specific blastomeres of early Xenopus embryos (4- and 8-cell stages) to study pronephric development.
To enable selective gene manipulation within the developing kidney lineage, minimizing off-target effects.

Main Methods:

Utilizing established Xenopus fate maps to identify specific blastomeres contributing to the pronephros.
Performing targeted microinjection of genetic material or lineage tracers into these identified blastomeres.
Employing whole-mount immunostaining at stages 38-40 to visualize pronephric development and assess cell contribution.

Main Results:

Successful verification of targeted microinjection into specific blastomeres using lineage tracers.
Assessment of the contribution of injected cells to the developing pronephros.
Demonstration of the protocol's adaptability for targeting other tissue types.

Conclusions:

The described microinjection technique provides a precise method for investigating pronephric development in Xenopus.
This protocol facilitates the study of kidney development and disease modeling through targeted gene manipulation.
The technique is versatile and can be applied to the study of various embryonic tissues in Xenopus.