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Published on: November 26, 2018
Circulating immune complexes (CIC) in Hodgkin's disease. I. Levels and two-dimensional analysis
A V Nerurkar1, S H Advani, B P Gothoskar
1Division of Biological Chemistry, Cancer Research Institute, Bombay, India.
Neoplasma
|January 1, 1989
Summary
Circulating immune complexes (CIC) are elevated in Hodgkin's disease (HD) patients, particularly those untreated. A specific 40 kD protein component in CICs may be quantitatively involved in HD pathogenesis.
Area of Science:
- Immunology
- Oncology
- Biochemistry
Background:
- Circulating immune complexes (CIC) play a role in various immune responses and diseases.
- Hodgkin's disease (HD) is a malignancy of the lymphatic system.
Purpose of the Study:
- To quantify CIC levels in patients with Hodgkin's disease (HD).
- To identify specific components within CICs that may be associated with HD.
Main Methods:
- Polyethylene glycol (PEG) mediated precipitation was used to isolate and quantify CICs from patient sera.
- Two-dimensional SDS-PAGE and peptide mapping were employed to analyze the protein components of CICs.
Main Results:
- Sera from untreated HD patients exhibited significantly elevated CIC levels compared to healthy donors.
- CIC levels decreased in patients who achieved clinical remission but remained above normal levels.
- HD patients in early stages (I and II) and with specific histology (LP and MC) showed preferential increases in CICs.
- A 40 kD protein component with a pI of 5.6 was found in significantly elevated amounts in CICs from untreated HD patients.
Conclusions:
- Elevated CIC levels are a hallmark of Hodgkin's disease.
- A specific 40 kD protein component within CICs is quantitatively associated with HD and may play a role in its pathogenesis.
- Monitoring CIC levels and specific components could offer insights into disease status and remission.
