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[Comparative proteomics study of different processing technology for pilose antler using iTRAQ technology coupled

Meng-ya Jin, Ling Dong, Yuan-ming Luo

Yao Xue Xue Bao = Acta Pharmaceutica Sinica

|May 13, 2016

Summary

Proteomics analysis of pilose antler processing reveals significant protein changes. Freeze-drying with trehalose enhances beneficial proteins, impacting platelet activation and spermatogonium maintenance.

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Area of Science:

Proteomics
Biochemistry
Food Science

Background:

Pilose antler is a valuable traditional medicine.
Processing methods significantly impact its biochemical composition.
Understanding these changes is crucial for quality control and therapeutic application.

Purpose of the Study:

To investigate the comparative proteomics of pilose antler processed using different methods.
To identify specific proteins affected by traditional frying process (TFP) and freeze-drying with protective agent (FDP).
To elucidate the functional implications of these protein alterations.

Main Methods:

iTRAQ technology coupled with 2D LC-MS/MS for protein identification and quantification.
MOLDI TOF/TOF mass spectrometry.
Bioinformatic analysis including Protein Pilot, Hierarchical clustering, and Gene Ontology (GO) for functional annotation.

Main Results:

1015 proteins identified; 87 differentially expressed proteins (DEPs) between TFP and fresh pilose antler.
7 significantly changed proteins (P ≤ 0.001) involved in calcium ion binding and ATP binding.
FDP (Trehalose) treatment increased significantly changed proteins, including Collagen alpha-1 (XII) chain (COL12A1) and Collagen alpha-1 (II) chain (COL2A1).
Key functions affected include platelet activation, spermatogonium maintenance, and tumor cell expression.

Conclusions:

Processing significantly alters the proteomic profile of pilose antler.
FDP with trehalose shows potential for preserving or enhancing beneficial proteins.
Identified proteins and pathways offer insights into the biological activities and therapeutic potential of processed pilose antler.