Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Upstream Processing01:27

Upstream Processing

16
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
16
Coordination of Gene Expression Processes in Bacteria01:29

Coordination of Gene Expression Processes in Bacteria

846
The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...
846
Repressible Operon: trp Operon01:21

Repressible Operon: trp Operon

2.3K
The trp operon in Escherichia coli exemplifies a repressible operon. It regulates the synthesis of tryptophan through repressor-mediated transcriptional control and attenuation. This dual regulatory mechanism ensures tryptophan biosynthesis occurs only when needed, conserving cellular resources.Structure of the trp OperonThe trp operon consists of five structural genes (trpE, trpD, trpC, trpB, and trpA) that encode enzymes for tryptophan biosynthesis. These genes are transcribed as a single...
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The dynamic growth of bacterial cultures: real-time Bayesian estimation of substrate uptake rates in fed-batch fermentations of E. coli.

Bioprocess and biosystems engineering·2025
Same author

The 10th Anniversary of <i>Bioengineering</i>: Biochemical Engineering.

Bioengineering (Basel, Switzerland)·2025
Same author

Addressing raw material variability: In-line FTIR sugar composition analysis of lignocellulosic process streams.

Bioresource technology·2024
Same author

Maduramycin, a novel glycosylation modulator for mammalian fed-batch and steady-state perfusion processes.

Journal of biotechnology·2024
Same author

Co-current filtrate flow in TFF perfusion processes: Decoupling transmembrane pressure from crossflow to improve product sieving.

Biotechnology and bioengineering·2023
Same author

Temperature Upshifts in Mammalian Cell Culture: A Suitable Strategy for Biosimilar Monoclonal Antibodies?

Bioengineering (Basel, Switzerland)·2023
Same journal

Bacterial degradation of aromatic ester pollutants in agro-ecosystems: implications for bioremediation.

Applied microbiology and biotechnology·2026
Same journal

Inhibiting biofilm growth on ammonium salt-functionalized or fluorinated voice prostheses silicone.

Applied microbiology and biotechnology·2026
Same journal

Enhancing reproducibility in mixing time determination of stirred tank reactors via automated analysis and standardized inter-laboratory trials.

Applied microbiology and biotechnology·2026
Same journal

Hybrid genome assembly and phenotypic assays reveal carbohydrate metabolism diversity in Lacticaseibacillus strains.

Applied microbiology and biotechnology·2026
Same journal

Ammonium and nitrate fluxes drive metabolic functional shifts in tap water biofilms.

Applied microbiology and biotechnology·2026
Same journal

Effects of controlled fermentation by Lactiplantibacillus plantarum PBS067 on Vitis vinifera L. leaf extract.

Applied microbiology and biotechnology·2026
See all related articles

Related Experiment Video

Updated: Mar 21, 2026

The Multifaceted Benefits of Protein Co-expression in Escherichia coli
12:48

The Multifaceted Benefits of Protein Co-expression in Escherichia coli

Published on: February 5, 2015

12.6K

Tunable recombinant protein expression in E. coli: enabler for continuous processing?

Lukas Marschall1, Patrick Sagmeister2, Christoph Herwig3,4

  • 1Institute of Chemical Engineering, Research Area Biochemical Engineering, Vienna University of Technology, Vienna, Austria.

Applied Microbiology and Biotechnology
|May 13, 2016
PubMed
Summary
This summary is machine-generated.

Expression tuning in Escherichia coli offers precise control over recombinant protein production. This technology overcomes challenges like toxicity and inclusion bodies, enabling efficient biopharmaceutical manufacturing.

Keywords:
All-or-none inductionContinuous processingE. coliTranscriptionTunable

More Related Videos

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
10:09

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Published on: June 27, 2017

14.0K
Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology
16:11

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

Published on: September 16, 2013

67.0K

Related Experiment Videos

Last Updated: Mar 21, 2026

The Multifaceted Benefits of Protein Co-expression in Escherichia coli
12:48

The Multifaceted Benefits of Protein Co-expression in Escherichia coli

Published on: February 5, 2015

12.6K
Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
10:09

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Published on: June 27, 2017

14.0K
Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology
16:11

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

Published on: September 16, 2013

67.0K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Bioprocess Engineering

Background:

  • Recombinant protein production in Escherichia coli faces challenges like toxicity, inclusion bodies, and metabolic burden due to suboptimal expression levels.
  • Tunable expression systems provide a technological solution to modulate recombinant protein expression, optimizing cellular resource utilization.

Purpose of the Study:

  • To review the genetic and process technological aspects of tunable expression systems in E. coli.
  • To provide a roadmap for the industrial application of these technologies.
  • To define "expression tuning" and highlight its applications.

Main Methods:

  • Review of genetic engineering strategies for tunable expression.
  • Analysis of process technological parameters influencing expression tuning.
  • Comparison of process strategies based on inducer type (metabolizable vs. non-metabolizable).

Main Results:

  • Tunable systems allow independent control of growth and expression rates, crucial for maximizing metabolic capacity.
  • Different process strategies are necessary depending on the inducer used for tuning.
  • Expression tuning addresses challenges like long-term culture stability and consistent product quality.

Conclusions:

  • Expression tuning is a key technology for efficient recombinant protein production in E. coli.
  • Independent control of growth and expression rates is essential for successful tuning.
  • Tunable expression systems are promising for continuous biopharmaceutical manufacturing.