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Updated: Mar 21, 2026

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
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Deciphering the epitranscriptome: A green perspective.

Alice Burgess1,2, Rakesh David1,2, Iain Robert Searle3,4,5

  • 1School of Biological Sciences, The University of Adelaide, South Australia,, 5005, Australia.

Journal of Integrative Plant Biology
|May 13, 2016
PubMed
Summary

RNA modifications, including N6-methyladenosine (m6A) and pseudouridine (Ψ), are crucial for plant development and stress responses. This review highlights key discoveries in the plant epitranscriptome, focusing on mRNA and non-coding RNA modifications.

Keywords:
ArabidopsisN6-methyladenosine (m6A)Pseudouridine (Ψ)RNA 5-methylcytosine (m5C)RNA modificationsepitranscriptome

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Area of Science:

  • Molecular Biology
  • Genetics
  • Plant Science

Background:

  • The epitranscriptome, a layer of gene regulation through RNA modifications, is increasingly recognized.
  • Over 100 RNA modifications exist, presenting a significant challenge for comprehensive understanding.
  • High-throughput sequencing and detection methods have advanced epitranscriptomic research.

Purpose of the Study:

  • To review current knowledge of RNA modifications, with a specific focus on plant discoveries.
  • To highlight the roles of various RNA modifications in plant development and stress responses.
  • To summarize recent findings on mRNA and non-coding RNA modifications in plants.

Main Methods:

  • Literature review of high-throughput sequencing and RNA modification detection studies.
  • Analysis of genetic mutation data in Arabidopsis affecting RNA modification enzymes.
  • Synthesis of research on specific RNA modifications like m6A, m5C, and Ψ.

Main Results:

  • RNA ribose modifications, base methylations (e.g., m6A, m5C), and pseudouridylation (Ψ) are essential for Arabidopsis development and stress tolerance.
  • These modifications impact RNA structure, stability, and translation efficiency.
  • Known modifications on transfer RNAs (tRNAs) and ribosomal RNAs (rRNAs) are well-characterized, with emerging research on mRNA and non-coding RNA modifications.

Conclusions:

  • RNA modifications play vital roles in regulating gene expression and biological processes in plants.
  • Further research is needed to elucidate the precise mechanisms of targeting and the specific functions of modified sites across different RNA classes.