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Updated: Mar 21, 2026

An Atmospheric Pressure Plasma Setup to Investigate the Reactive Species Formation

An Atmospheric Pressure Plasma Setup to Investigate the Reactive Species Formation

Published on: November 3, 2016

Stabilizing the cold plasma-stimulated medium by regulating medium's composition.

Dayun Yan¹, Niki Nourmohammadi², Ka Bian³

¹Department of Mechanical and Aerospace Engineering, The George Washington University, Science &Engineering Hall, 800 22nd Street, NW, Room 3550, Washington, DC 20052, USA.

Scientific Reports

|May 14, 2016

Summary

This summary is machine-generated.

Cold plasma-stimulated medium (PSM) shows anti-cancer effects but degrades during storage. This study identifies amino acid reactions as the cause and proposes stabilization methods for broader cancer treatment applications.

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Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells

Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells

Published on: November 17, 2017

Area of Science:

Biomedical Engineering
Cancer Research
Plasma Medicine

Background:

Cold plasma-stimulated medium (PSM) offers a flexible platform for cancer treatment, comparable to direct plasma irradiation.
A significant limitation of PSM is its degradation during storage, necessitating freezing at -80°C for stability.
Understanding PSM degradation is crucial for developing practical, room-temperature-stable cancer therapies.

Purpose of the Study:

To identify the primary cause of PSM degradation during storage.
To develop methods for stabilizing PSM at higher temperatures (8°C and -25°C).
To preserve both hydrogen peroxide (H2O2) levels and anti-cancer efficacy of PSM during storage.

Main Methods:

Investigated the reaction between reactive species in PSM and specific amino acids.
Tested stabilization using phosphate-buffered saline (PBS) and modified Dulbecco's Modified Eagle Medium (DMEM) lacking cysteine and methionine.
Evaluated the effect of adding 3-Nitro-L-tyrosine to DMEM on PSM stability.

Main Results:

PSM degradation is primarily caused by reactions between reactive species and cysteine/methionine amino acids.
Using PBS and cysteine/methionine-free DMEM significantly stabilized PSM at 8°C and -25°C for at least 3 days.
Addition of 3-Nitro-L-tyrosine further mitigated PSM degradation at 8°C for 3 days.

Conclusions:

Identified key amino acids responsible for PSM instability.
Developed practical storage stabilization strategies for PSM, enabling higher temperature storage.
This research lays the groundwork for the future clinical application of stabilized PSM in cancer therapy.