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Assessing Tumor Angiogenesis in Histological Samples.

Jia-Min Pang¹, Nicholas Jene¹, Stephen B Fox²

¹Department of Pathology, Peter MacCallum Cancer Centre, 2 St Andrews Place, Melbourne, 3002, Australia.

Methods in Molecular Biology (Clifton, N.J.)

|May 14, 2016

Summary

Tumor neovascularization involves complex vessel formation processes. Histological assessment of tumor vascularity, while mainly for research, shows potential clinical applications with standardized methods.

Keywords:

Angiogenic factors Chalkley counts Hypoxia Microvessel density Tumor angiogenesis Vascular grading

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Published on: November 23, 2014

Area of Science:

Oncology
Vascular Biology
Histopathology

Background:

Tumor neovascularization is crucial for tumor growth and metastasis.
Tumor vessels form via angiogenesis, vasculogenesis, and other mechanisms.
Understanding tumor vascularity aids in developing anti-cancer therapies.

Purpose of the Study:

To review the processes of tumor neovascularization.
To discuss methods for quantifying tumor vascularity.
To highlight molecular markers and reagents for studying tumor vasculature.

Main Methods:

Quantification of microvessels using immunohistochemistry (hot spot counting).
Advanced techniques like Chalkley counting and image analysis systems.
Utilizing molecular markers for angiogenic factors, cell adhesion molecules, and hypoxia.

Main Results:

Identified multiple pathways governing tumor neovascularization.
Developed reagents to study molecular aspects of tumor vasculature.
Established methods to differentiate active from quiescent vessels.

Conclusions:

Histological assessment of tumor vascularity is primarily a research tool.
Standardized methodologies and trained observers could enable clinical applications.
Further research may translate histological findings into clinical practice.