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Related Experiment Video

Updated: Mar 21, 2026

Endothelial Cell Tube Formation Assay for the In Vitro Study of Angiogenesis
08:12

Endothelial Cell Tube Formation Assay for the In Vitro Study of Angiogenesis

Published on: September 1, 2014

62.2K

Tube-Forming Assays.

Ryan M Brown1, Christopher J Meah2, Victoria L Heath2

  • 1Centre for Doctoral Training in Physical Sciences of Imaging in the Biomedical Sciences and School of Chemistry, University of Birmingham, Birmingham, UK.

Methods in Molecular Biology (Clifton, N.J.)
|May 14, 2016
PubMed
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This study details the Matrigel tube-forming assay for studying angiogenesis, or new blood vessel growth. An ImageJ plugin is introduced for automated analysis, improving efficiency and objectivity in angiogenesis research.

Area of Science:

  • Biomedical Sciences
  • Cell Biology
  • Vascular Biology

Background:

  • Angiogenesis, the formation of new blood vessels, is a critical biological process.
  • It is regulated by numerous growth factors and signaling pathways.
  • In vivo studies of angiogenesis are complex, necessitating reliable experimental assays.

Purpose of the Study:

  • To provide a detailed protocol for the Matrigel tube-forming assay.
  • To review methods for analyzing tube formation in angiogenesis studies.
  • To introduce an automated ImageJ plugin for quantifying tube formation.

Main Methods:

  • Implementation of the Matrigel tube-forming assay.
  • Review of established methods for analyzing angiogenesis.
  • Development and application of an ImageJ plugin for automated image quantification.
Keywords:
AngiogenesisEndothelial cellImageJMatrigelSproutingTube formation

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Microtiter Dish Biofilm Formation Assay
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Related Experiment Videos

Last Updated: Mar 21, 2026

Endothelial Cell Tube Formation Assay for the In Vitro Study of Angiogenesis
08:12

Endothelial Cell Tube Formation Assay for the In Vitro Study of Angiogenesis

Published on: September 1, 2014

62.2K
A Matrigel-Based Tube Formation Assay to Assess the Vasculogenic Activity of Tumor Cells
04:00

A Matrigel-Based Tube Formation Assay to Assess the Vasculogenic Activity of Tumor Cells

Published on: September 7, 2011

67.5K
Microtiter Dish Biofilm Formation Assay
03:57

Microtiter Dish Biofilm Formation Assay

Published on: January 30, 2011

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Main Results:

  • The Matrigel assay provides a robust model for studying angiogenesis.
  • The ImageJ plugin significantly reduces analysis time and subjectivity.
  • Automated quantification enhances the reliability of results from tube-forming assays.

Conclusions:

  • The Matrigel tube-forming assay is a valuable tool for angiogenesis research.
  • Automated analysis using the ImageJ plugin improves experimental efficiency and data accuracy.
  • This combined approach facilitates the identification of biomolecules involved in angiogenesis.