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Related Concept Videos

Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Related Experiment Video

Updated: Mar 21, 2026

Propagating and Detecting an Infectious Molecular Clone of Maedi-visna Virus that Expresses Green Fluorescent Protein
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A replicating plasmid-based vector for GFP expression in Mycoplasma hyopneumoniae.

H Z A Ishag1,2, M J Liu1, R S Yang1

  • 1Key Laboratory of Veterinary Biological Engineering and Technology, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Ministry of Agriculture, National Research Center for Engineering and Technology of Veterinary Bio-Products, Nanjing, China.

Genetics and Molecular Research : GMR
|May 14, 2016
PubMed
Summary

Researchers developed a GFP reporter system for Mycoplasma hyopneumoniae, enabling genetic manipulation. This breakthrough facilitates functional genomic analyses for the pathogen causing porcine enzootic pneumonia.

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Area of Science:

  • Veterinary Microbiology
  • Molecular Biology

Background:

  • Mycoplasma hyopneumoniae causes significant economic losses in the global swine industry due to porcine enzootic pneumonia (PEP).
  • Limited genetic tools have hindered in-depth studies of M. hyopneumoniae pathogenesis despite whole-genome sequencing.

Purpose of the Study:

  • To develop a general GFP reporter vector for Mycoplasma hyopneumoniae.
  • To establish a feasible method for genetic manipulation and gene expression analysis in M. hyopneumoniae.

Main Methods:

  • Construction of an expression plasmid (pMD18-TOgfp) containing the p97 gene promoter, origin of replication, tetracycline resistance marker, and GFP gene.
  • Transformation of competent M. hyopneumoniae (strain 168L) using the constructed plasmid.
  • Optimization of an electroporation protocol for efficient transformation.

Main Results:

  • Successful expression of the GFP reporter gene in M. hyopneumoniae transformants, confirmed by fluorescence microscopy.
  • Demonstration that the p97 gene promoter drives GFP expression in M. hyopneumoniae.
  • Optimization of an electroporation method yielding an efficiency of approximately 1 x 10^-6 transformants/μg plasmid DNA.

Conclusions:

  • Mycoplasma hyopneumoniae is susceptible to genetic manipulation, allowing for foreign gene expression.
  • The developed GFP reporter system provides a valuable tool for functional genomic studies in M. hyopneumoniae.
  • This advancement is expected to spur the development of further genetic tools for M. hyopneumoniae research.