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Updated: Mar 21, 2026

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

Published on: May 19, 2018

Transcript analysis of a goat mesenteric lymph node by deep next-generation sequencing.

G X E¹, Y J Zhao¹, R S Na¹

¹Chongqing Key Laboratory of Forage & Herbivore, Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization, College of Animal Science and Technology, Southwest University, Chongqing, China.

Genetics and Molecular Research : GMR

|May 14, 2016

Summary

This summary is machine-generated.

Deep RNA sequencing of goat lymph nodes revealed a complex transcriptome. Juvenile goats show an immature immune system, highlighting the need for further functional annotation in non-model organisms.

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Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

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Published on: May 19, 2018

Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing

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T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Published on: January 12, 2021

Area of Science:

Genomics
Immunology
Veterinary Science

Background:

Functional annotation of non-model mammalian genomes, like goats, is limited.
Deep RNA sequencing (RNA-seq) offers a cost-effective method for genomic exploration in non-model organisms.

Purpose of the Study:

To comprehensively characterize the transcript catalog of goat intestinal mucous membrane lymph nodes.
To compare mRNA expression between juvenile and post-adolescent goats.

Main Methods:

Whole transcriptome analysis using Illumina HiSeq 4000.
Acquisition of 9.692 GB of raw reads.
Mapping of 57,526 lymph transcripts to known units.

Main Results:

8949 differentially expressed transcripts identified between juvenile and post-adolescent stages, including 6174 known genes.
Functional classification using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) terms.
Identification of immune-related pathways, suggesting immaturity in juvenile goat mesenteric lymph nodes.

Conclusions:

The study reveals a complex transcriptome profile in goat lymph nodes.
Findings suggest an immature immune system in juvenile goats' mesenteric lymph nodes.
Highlights the importance of RNA-seq for non-model organism genomic annotation.