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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
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Post-SELEX optimization of aptamers.

Shunxiang Gao1, Xin Zheng2, Binghua Jiao3,4

  • 1Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Second Military Medical University, Shanghai, 200433, China.

Analytical and Bioanalytical Chemistry
|May 14, 2016
PubMed
Summary
This summary is machine-generated.

Aptamers, or nucleic acid ligands, offer advantages over antibodies but face challenges. Post-SELEX optimization strategies like truncation and chemical modification enhance aptamer properties for broader applications.

Keywords:
AptamerChemical modificationMutationOptimizationPolyvalent ligandTruncation

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Drug Development

Background:

  • Aptamers are single-stranded DNA/RNA selected via SELEX, binding targets with high affinity and specificity.
  • Despite advantages over antibodies (stability, modification), few aptamers reach clinical use due to issues like nuclease instability and low affinity.

Purpose of the Study:

  • To review recent post-SELEX optimization strategies for aptamers.
  • To detail methods that improve aptamer properties for practical applications.

Main Methods:

  • Focuses on post-SELEX optimization techniques.
  • Details four common strategies: truncation, chemical modification, bivalent/multivalent construction, and mutagenesis.

Main Results:

  • These strategies aim to enhance specific aptamer properties such as stability, affinity, and bioavailability.
  • Optimization is tailored to the intended application of the aptamer.

Conclusions:

  • Post-SELEX optimization is crucial for overcoming limitations of aptamers.
  • Tailored strategies can significantly improve aptamer performance for clinical and research use.