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Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
Published on: September 5, 2013
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Complete Genome Sequence of Staphylococcus aureus MCRF184, a Necrotizing Fasciitis-Causing Methicillin-Sensitive
Vijay Aswani1, Bob Mau2, Sanjay K Shukla3
1Department of Internal Medicine and Pediatrics, Marshfield Clinic, Marshfield, Wisconsin, USA.
Genome Announcements
|May 14, 2016
Summary
We sequenced the genome of a highly virulent methicillin-sensitive Staphylococcus aureus strain, MCRF184. This strain, identified as sequence type 45, was isolated from a patient with necrotizing fasciitis.
Area of Science:
- Microbiology
- Genomics
- Infectious Diseases
Background:
- Staphylococcus aureus is a significant human pathogen.
- Certain strains exhibit high virulence and cause severe infections like necrotizing fasciitis.
- Understanding the genetic basis of virulence is crucial for developing effective treatments.
Purpose of the Study:
- To report the complete genome sequence of a highly virulent methicillin-sensitive Staphylococcus aureus (MSSA) strain.
- To characterize the genetic features of MSSA MCRF184, isolated from a necrotizing fasciitis patient.
Main Methods:
- Whole-genome sequencing of the Staphylococcus aureus strain MCRF184.
- Bioinformatic analysis to determine sequence type and identify virulence-associated genes.
Main Results:
- The complete genome sequence of MSSA strain MCRF184 was obtained.
- The strain belongs to sequence type 45 (ST45).
- The isolate originated from a surgical biopsy of a patient diagnosed with necrotizing fasciitis.
Conclusions:
- The genome sequence provides a valuable resource for studying the virulence mechanisms of ST45 Staphylococcus aureus.
- This data can aid in understanding the pathogenesis of severe infections caused by this pathogen.
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