Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cooperative Allosteric Transitions

Cooperative Allosteric Transitions

Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...

Cooperative Allosteric Transitions

Cooperative Allosteric Transitions

Cooperative Allosteric Transitions

Cooperative Allosteric Transitions

Cooperative Binding of Transcription Regulators

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...

Cooperative Binding of Transcription Regulators

Cooperative Binding of Transcription Regulators

Negative Regulator Molecules

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Dietary effects of black soldier fly oil, soybean oil, and tallow on performance, egg traits, hormones, and oviductal connexins in laying hens.

Animal : an international journal of animal bioscience·2026

Same author

Association of Left Vertebral Artery Hypoplasia with Posterior Circulation Stroke and the Functional Outcome of Patients with Atrial Fibrillation-Related Cardioembolic Stroke.

AJNR. American journal of neuroradiology·2022

Same author

Induction of cell cycle arrest and apoptosis by tomentosin in hepatocellular carcinoma HepG2 and Huh7 cells.

Human & experimental toxicology·2020

Same author

The organ-specific differential roles of rice DXS and DXR, the first two enzymes of the MEP pathway, in carotenoid metabolism in Oryza sativa leaves and seeds.

BMC plant biology·2020

Same author

Effect of Dietary sugar beet pulp supplementation on growth performance, nutrient digestibility, fecal Microflora, blood profiles and Diarrhea incidence in weaning pigs.

Journal of animal science and technology·2017

Same author

Various levels of copra meal supplementation with <i>β</i>-Mannanase on growth performance, blood profile, nutrient digestibility, pork quality and economical analysis in growing-finishing pigs.

Journal of animal science and technology·2017

Same journal

Erratum for the Research Article "Detecting supramolecular organic nanoparticles during heat wave".

Science (New York, N.Y.)·2026

Same journal

Local signals, systemic decline.

Science (New York, N.Y.)·2026

Same journal

The mechanics of liver regeneration.

Science (New York, N.Y.)·2026

Same journal

Computing in a memory with physics.

Science (New York, N.Y.)·2026

Same journal

Retraction.

Science (New York, N.Y.)·2026

Same journal

Making time.

Science (New York, N.Y.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 21, 2026

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors

Published on: August 17, 2019

Thresholds and ultrasensitivity from negative cooperativity.

S H Ha¹, J E Ferrell²

¹Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, CA 94305-5174, USA.

Science (New York, N.Y.)

|May 14, 2016

Summary

This summary is machine-generated.

Negative cooperativity in receptor binding can create highly ultrasensitive biological responses with thresholds, especially when ligands bind with high affinity. This mechanism is crucial for complex biological behaviors like bistability and oscillations.

More Related Videos

Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b

Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b

Published on: November 11, 2016

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

Published on: September 13, 2014

Related Experiment Videos

Last Updated: Mar 21, 2026

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors

Published on: August 17, 2019

Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b

Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b

Published on: November 11, 2016

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

Published on: September 13, 2014

Area of Science:

Biochemistry
Systems Biology
Molecular Biology

Background:

Negative cooperativity describes reduced ligand binding affinity after initial binding to multimeric receptors.
This phenomenon typically results in a more graded receptor response.

Purpose of the Study:

To investigate the impact of negative cooperativity on receptor-ligand dynamics under specific conditions.
To explore the potential for negative cooperativity to generate ultrasensitive biological responses.

Main Methods:

Theoretical modeling of receptor-ligand interactions.
Analysis of experimental results concerning binding affinity and ligand depletion.

Main Results:

Negative cooperativity, combined with high ligand affinity and significant ligand depletion, produces a highly ultrasensitive receptor response.
This ultrasensitivity is characterized by a pronounced threshold effect.

Conclusions:

Negative cooperativity can drive qualitatively different biological responses beyond simple graded changes.
The interplay of negative cooperativity, high affinity, and ligand depletion is critical for generating ultrasensitivity and thresholds.
These ultrasensitive responses are fundamental to complex biological systems, including bistability and oscillations, highlighting the importance of negative cooperativity in biological regulation.