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MicroRNA‑126 is downregulated in thyroid cancer cells, and regulates proliferation, migration and invasion by

Yan Qian1, Xiaoli Wang2, Zhanlu Lv1

  • 1Department of Environmental Pollution and Health, State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, P.R. China.

Molecular Medicine Reports
|May 14, 2016
PubMed

Insights

MicroRNA-126 (miR-126) is downregulated in thyroid cancer, inhibiting cancer cell proliferation, migration, and invasion. This suggests miR-126 could be a potential therapeutic target for thyroid cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNA-126 (miR-126) is known to be downregulated in several cancers.
  • Its role in human thyroid cancer has not been previously investigated.

Purpose of the Study:

  • To determine the expression levels of miR-126 in human thyroid cancer.
  • To investigate the functional effects of miR-126 on thyroid cancer cell behavior.
  • To identify potential molecular targets of miR-126 in thyroid cancer.

Main Methods:

  • Quantitative polymerase chain reaction (qPCR) to measure miR-126 expression in tumor tissues and cell lines.
  • Cell proliferation, migration, and invasion assays.
  • Western blot analysis and luciferase assays to confirm direct targeting of C-X-C chemokine receptor type 4 (CXCR4).

Main Results:

  • miR-126 expression was significantly downregulated in thyroid cancer tissues and cell lines compared to normal controls.
  • Upregulation of miR-126 using mimics suppressed thyroid cancer cell proliferation, migration, and invasion.
  • miR-126 was found to directly target CXCR4 in thyroid cancer cells.

Conclusions:

  • miR-126 plays an inhibitory role in thyroid cancer progression.
  • The findings suggest that miR-126, by targeting CXCR4, could serve as a novel therapeutic target for thyroid cancer.

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