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Studies of Panax japonicus fibrinolysis
Abstract:
The effects of a 70% methanol extract (PMe) obtained from the rhizomes of Panax japonicus C. A. Meyer on experimental thrombosis and fibrinolysis were investigated in vivo and in vitro. PMe showed a promotive effect on the activation of the fibrinolytic system as determined by the euglobulin lysis time (ELT) assay but was inactive to the inhibitory effect against endotoxin-induced disseminated intravascular coagulation (DIC) in rats. PMe and its major components, chikusetsusaponin III, IV, and V, strongly promoted the action of urokinase in fibrin plate. These results suggested that PMe promotes the fibrinolysis and its effective components are chikusetsusaponin III, IV, and V.
Insights
Panax japonicus methanol extract (PMe) enhances fibrinolysis, promoting clot breakdown. Its active components, chikusetsusaponins III, IV, and V, boost urokinase activity, suggesting therapeutic potential for thrombosis.
Area of Science:
- Pharmacology
- Biochemistry
- Traditional Medicine
Background:
- Thrombosis is a major cause of cardiovascular disease.
- Fibrinolysis, the breakdown of blood clots, is crucial for vascular health.
- Panax japonicus is a traditional medicinal herb with potential therapeutic properties.
Purpose of the Study:
- To investigate the effects of Panax japonicus methanol extract (PMe) on thrombosis and fibrinolysis.
- To identify the active components within PMe responsible for these effects.
Main Methods:
- In vivo and in vitro experiments were conducted.
- Euglobulin lysis time (ELT) assay was used to assess fibrinolytic activity.
- Assays were performed to evaluate inhibitory effects against endotoxin-induced disseminated intravascular coagulation (DIC).
- Fibrin plate assays were used to determine the effect on urokinase activity.
Main Results:
- PMe demonstrated a significant promotive effect on the fibrinolytic system, as indicated by shortened ELT.
- PMe was found to be inactive in inhibiting endotoxin-induced DIC in rats.
- PMe and its major components (chikusetsusaponins III, IV, and V) strongly promoted urokinase activity in vitro.
Conclusions:
- PMe effectively promotes fibrinolysis, suggesting a role in clot dissolution.
- Chikusetsusaponins III, IV, and V are identified as the key active components responsible for PMe's fibrinolytic effects.
- These findings support the potential of PMe and its components in managing thrombotic disorders.