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    We developed a portable interferometric module for quantitative phase microscopy of live cells. This simple, lens-free device enables straightforward imaging of dynamic cellular processes in microfluidic channels.

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    Area of Science:

    • Biophysics
    • Optical Microscopy
    • Cell Biology

    Background:

    • Quantitative phase microscopy (QPM) provides label-free imaging of cell morphology and dynamics.
    • Existing QPM systems can be complex, bulky, and difficult to align.
    • There is a need for portable and user-friendly QPM solutions for live-cell imaging.

    Purpose of the Study:

    • To present a novel, portable, off-axis interferometric module for QPM.
    • To demonstrate its utility for imaging live cells in microfluidic devices.
    • To simplify the setup and alignment of QPM systems.

    Main Methods:

    • An off-axis interferometric module was designed and integrated with an inverted microscope.
    • The module utilizes a modified Michelson interferometer with a retro-reflector.
    • It generates an interference pattern between the sample image and its flipped version.
    • No lenses, pinholes, or gratings are incorporated, simplifying alignment.

    Main Results:

    • The module was successfully implemented as a portable add-on for QPM.
    • Straightforward alignment and full control of the off-axis angle were achieved.
    • The system demonstrated effective imaging of live cells in a micro-channel.
    • Ghost images were successfully avoided.

    Conclusions:

    • The developed interferometric module offers a simplified and portable solution for QPM.
    • It is suitable for label-free, quantitative imaging of live cells, particularly in microfluidic applications.
    • The lens-free design and straightforward alignment facilitate broader adoption of QPM techniques.