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Tracking Effector and Memory NK Cells During MCMV Infection.

Aimee M Beaulieu1, Joseph C Sun2

  • 1Department of Microbiology, Biochemistry and Moleculor Genetics, Center for Immunity and Inflammation, Rutgers New Jersey Medical School, New York, NJ, 07103, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 15, 2016
PubMed
Summary

Natural killer (NK) cells expressing Ly49H receptors expand during mouse cytomegalovirus (MCMV) infection, contract, and form long-lived memory cells. This study tracks these Ly49H(+) NK cells using an adoptive transfer model in C57BL/6 mice.

Keywords:
Innate lymphocytesMCMVMemoryNK cells

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Area of Science:

  • Immunology
  • Virology
  • Cellular immunology

Background:

  • NK cells expressing the activating receptor Ly49H undergo significant expansion following mouse cytomegalovirus (MCMV) infection in C57BL/6 mice.
  • This expansion is followed by a contraction phase, resulting in a persistent population of memory-like NK cells.

Purpose of the Study:

  • To establish and utilize an adoptive transfer model to track Ly49H(+) NK cell dynamics during MCMV infection.
  • To investigate the expansion, contraction, and long-term persistence of MCMV-experienced Ly49H(+) NK cells.

Main Methods:

  • Adoptive transfer of Ly49H(+) NK cells into congenic C57BL/6 hosts.
  • Infection of recipient mice with mouse cytomegalovirus (MCMV).
  • Monitoring and quantification of transferred Ly49H(+) NK cell populations post-infection.

Main Results:

  • The study successfully tracked the proliferation and contraction of transferred Ly49H(+) NK cells in response to MCMV.
  • Evidence of a long-lived pool of MCMV-experienced Ly49H(+) NK cells was observed.
  • These memory NK cells demonstrated potential for enhanced responses upon re-exposure.

Conclusions:

  • An adoptive transfer model provides a robust system for studying NK cell memory formation after viral infection.
  • Ly49H(+) NK cells exhibit a characteristic expansion-contraction-persistence pattern following MCMV infection.
  • MCMV-induced NK cell memory is a key feature of adaptive immunity to this virus.