Related Experiment Video
Updated: Mar 21, 2026

07:20
Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins
Published on: June 14, 2018
13.2K
Electroporation of siRNA to Silence Gene Expression in Primary NK Cells
Prasad V Phatarpekar1, Dean A Lee1, Srinivas S Somanchi2
1Division of Pediatrics, The University of Texas, M.D. Anderson Cancer Center, Houston, TX, 77030, USA.
Methods in Molecular Biology (Clifton, N.J.)
|May 15, 2016
Abstract:
Gene silencing through siRNA is an effective experimental tool to unravel molecular mechanisms involved in cellular processes. Here we describe a method to silence gene expression in primary human natural killer (NK) cells by transfecting ON-TARGETplus SMART pool siRNA using an electroporation-based method called Nucleofection(®). The technique yields effective silencing of the target gene without any off-target effects.

