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Related Experiment Video

Updated: Mar 21, 2026

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides

Published on: November 24, 2010

Non-functional genes repaired at the RNA level.

Gertraud Burger¹

¹Department of Biochemistry, Robert-Cedergren Centre for Bioinformatics and Genomics, Université de Montréal, Montréal, Canada.

Comptes Rendus Biologies

|May 16, 2016

Summary

This summary is machine-generated.

Gene evolution involves aberrations, but some organisms like diplonemids use RNA-level repair and editing to restore function in fragmented genes. This unique system allows their survival.

Keywords:

Diplonemids Diplonémides Evolution of complexity Gene fragmentation Gènes fragmentés Insertion and substitution RNA editing Protistes Protists Trans-splicing Édition de l’ARN par insertion et substitution Épissage en trans Évolution de la complexité

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Published on: August 25, 2017

Area of Science:

Molecular Biology
Genetics
Evolutionary Biology

Background:

Gene sequences are subject to constant evolutionary changes including substitutions, insertions, deletions, and chromosomal rearrangements.
Organisms employ repair mechanisms at the gene or product level to maintain functionality and ensure survival.
Diplonemids, a poorly studied protist group, exhibit unique genetic characteristics.

Purpose of the Study:

To introduce common types of gene aberrations and their repair mechanisms.
To focus on the systematically fragmented genes in diplonemids.
To explore the evolutionary origins and persistence of the diplonemid system.

Main Methods:

Review of common gene aberration types and repair strategies.
Analysis of gene fragmentation in diplonemids.
Investigation of RNA-level gene expression and RNA editing in diplonemids.

Main Results:

Gene fragmentation is a common occurrence due to various evolutionary pressures.
Diplonemids express fragmented genes through RNA splicing and extensive RNA editing.
RNA editing in diplonemids compensates for point mutations in fragmented genes.

Conclusions:

Diplonemids possess a highly unorthodox gene expression system involving RNA-level restitching and editing.
This unique system contributes to the survival and thriving of diplonemids over millions of years.
The evolutionary pathways leading to this system are complex and warrant further investigation.