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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Mar 21, 2026

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

Multiplexed labeling system for high-throughput cell sorting.

Seung Won Shin¹, Kyung Soo Park¹, In Hyun Song¹

¹School of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, South Korea.

Analytical Biochemistry

|May 17, 2016

Summary

This summary is machine-generated.

Researchers developed DNA nanostructures for multiplexed fluorescent cell analysis. This technique uses two dyes to create seven distinct fluorescent codes, enhancing flow cytometry efficiency for bioanalysis.

Keywords:

Cell sorting DNA nanostructure Fluorescence-activated cell sorting Multilabeled fluorescence marker

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Area of Science:

Biotechnology
Molecular Biology
Analytical Chemistry

Background:

Flow cytometry and cell sorting require multiplexed fluorescent labeling for simultaneous analysis of multiple cell phenotypes.
Current methods for generating multiple fluorescent colors are limited, especially with restricted laser sources, hindering efficient multiplexed detection.

Purpose of the Study:

To develop a novel method for generating multiple fluorescent colors using DNA nanostructures for enhanced multiplexed cell analysis.
To demonstrate the capability of DNA nanostructures as platforms for creating complex fluorescent codes.

Main Methods:

Utilized self-assembly of fluorescent dye-conjugated oligonucleotides to create anisotropic DNA nanostructures, specifically Y-shaped DNA (Y-DNA) and tree-shaped DNA (T-DNA).
Employed T-DNA nanostructures as platforms to generate multiple fluorescent codes by complexing two different fluorescent dyes.

Main Results:

Successfully demonstrated the creation of seven distinct fluorescent codes using only two different fluorescent dyes with T-DNA nanostructures.
The developed DNA nanostructure-based system enables highly efficient multiplexed fluorescent detection.

Conclusions:

DNA nanostructures offer a versatile platform for generating complex fluorescent codes, significantly improving multiplexing capabilities in flow cytometry.
This approach enhances the efficiency of bioanalysis by enabling more sophisticated fluorescent detection compared to traditional methods.