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[Embryo transfer in swine in relation to a gene transfer program].
1Institut für Tierzucht und Tierhygiene der Ludwig-Maximilians-Universität München.
Tierarztliche Praxis. Supplement
|January 1, 1989
Summary
Optimizing donor pig preparation is crucial for successful DNA microinjection into pig embryos. This study details a protocol, highlighting that centrifugation and microinjection significantly reduce embryo survival rates.
Area of Science:
- Reproductive biology
- Genetics
- Animal science
Context:
- Efficient production of genetically modified pigs requires precise embryonic stage selection for DNA microinjection.
- Established protocols for oestrus induction and ovulation in donor pigs using PMSG and HCG hormones.
Purpose:
- To detail a protocol for preparing donor pigs for DNA microinjection into pronuclei.
- To establish an in vivo culture system to assess microinjection's impact on embryo survival.
- To evaluate the effects of centrifugation and microinjection on pig zygote viability.
Summary:
- A precise protocol using PMSG and HCG for donor pig preparation was employed.
- Eggs were collected, centrifuged, and subjected to DNA microinjection, with survival rates noted at each step.
- An in vivo oviduct culture system was used to measure the impact of microinjection procedures on embryo survival.
Impact:
- The study quantifies significant reductions in embryo survival post-centrifugation (38%) and microinjection (12%).
- Identified a relationship between donor pig body weight and egg recovery rate and quality.
- Achieved a 37% pregnancy rate with an average of 3.9 piglets per litter after embryo transfer, demonstrating the feasibility of the protocol.