[Effect and Regulatory Mechanism of Clock Gene Per1 on Biological Behaviors of Human Oral Squamous Carcinoma Cell]

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Updated: Mar 21, 2026

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
06:53

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Published on: November 11, 2016

8.9K

Han-xue Li1, Kai Yang1, Xiao-juan Fu1

  • 1Department of Oral and Maxillofacial Surgery,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China;

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
|May 17, 2016
PubMed
Abstract

Insights

The clock gene Period 1 (Per1) knockdown promotes oral cancer cell growth and spread by affecting key genes involved in proliferation, apoptosis, and invasion. Further study of Per1 offers potential new cancer treatment targets.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Chronobiology

Background:

  • The circadian clock gene Period 1 (Per1) plays a role in cellular processes.
  • Aberrant Per1 expression is implicated in various cancers.
  • Understanding Per1's function in oral squamous cell carcinoma is crucial.

Purpose of the Study:

  • To investigate the role and regulatory mechanism of the clock gene Per1.
  • To determine Per1's effect on proliferation, apoptosis, migration, and invasion.
  • To analyze Per1's impact on key gene expression in human oral squamous carcinoma SCC15 cells.

Main Methods:

  • RNA interference (RNAi) was used to knockdown Per1 in SCC15 cells.
  • Flow cytometry assessed cell proliferation and apoptosis.
  • Transwell assays evaluated cell migration and invasion.
  • Real-time PCR measured mRNA expression of target genes (Ki-67, MDM2, c-Myc, p53, Bax, Bcl-2, MMP2, MMP9, VEGF).

Main Results:

  • Per1 knockdown significantly promoted SCC15 cell proliferation, migration, and invasion (P<0.05).
  • Per1 knockdown inhibited SCC15 cell apoptosis (P<0.05).
  • Per1 knockdown altered mRNA expression of Ki-67, MDM2, Bcl-2, MMP2, MMP9, c-Myc, p53, and Bax, but not VEGF.

Conclusions:

  • Clock gene Per1 regulates downstream tumor-related genes, influencing oral cancer cell behavior.
  • Aberrant Per1 expression impacts tumor cell proliferation, apoptosis, migration, and invasion.
  • Per1 presents a potential molecular target for novel cancer therapies.

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