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Related Experiment Video

Updated: Mar 21, 2026

Generation of Cancer Cell Clones to Visualize Telomeric Repeat-containing RNA TERRA Expressed from a Single Telomere in Living Cells
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Translin and Trax differentially regulate telomere-associated transcript homeostasis.

Natalia Gomez-Escobar1, Nasser Almobadel1, Othman Alzahrani1

  • 1North West Cancer Research Institute, School of Medical Sciences, Bangor University, Bangor, Gwynedd, United Kingdom.

Oncotarget
|May 18, 2016
PubMed
Summary
This summary is machine-generated.

Translin (Tsn1) and Trax (Tfx1) proteins regulate telomere-associated transcripts in fission yeast and human cells. They control telomeric repeat-associated sequences (TERRAs) and sub-telomeric transcripts through a reciprocal mechanism.

Keywords:
C3POChromosome SectionTERRATranslinTraxtelomeres

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Area of Science:

  • Molecular Biology
  • Genetics
  • Epigenetics

Background:

  • Translin and Trax are conserved nucleic acid-binding proteins involved in RNA regulation.
  • Their roles in processes like RNA interference and microRNA degradation are known.
  • Their specific function in regulating telomeric transcripts remained unexplored.

Purpose of the Study:

  • To investigate the function of Translin and Trax proteins in fission yeast.
  • To elucidate the mechanism by which these proteins control telomere-associated transcripts.
  • To determine if this mechanism is conserved in human cells.

Main Methods:

  • Transcript analysis in fission yeast.
  • Genetic mutation studies of tfx1+ (Trax) and Tsn1 (Translin).
  • Comparative analysis in human cell lines.

Main Results:

  • Mutation of tfx1+ (Trax) increases transcript levels from silenced sub-telomeric regions.
  • Tsn1 (Translin) is partially required for this elevation.
  • Tsn1 (Translin) represses telomeric repeat-associated sequences (TERRAs), while Tfx1 maintains their elevated levels.
  • Human Translin and Trax also regulate telomere-associated transcripts in a telomere-specific manner.

Conclusions:

  • A novel, reciprocal mechanism for telomere-associated transcript regulation by Translin and Trax has been identified.
  • This regulatory mechanism is conserved between fission yeast and human cells.
  • Translin and Trax play a crucial role in maintaining telomere stability and function through RNA regulation.