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A very small viral double-stranded RNA.

T H Chang1, N Banerjee, J Bruenn

  • 1Department of Biology, California Institute of Technology, Pasadena 91125.

Virus Genes
|March 1, 1989
PubMed
Summary
This summary is machine-generated.

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The Ustilago maydis virus (UmV) P1 L dsRNA segment is derived from the 3' end of the M segment. This unique process involves replicating and packaging a processed mRNA fragment, unlike other dsRNA viruses.

Area of Science:

  • Virology
  • Mycology
  • Molecular Biology

Background:

  • Ustilago maydis (UmV) is a double-stranded RNA virus infecting the corn fungal pathogen Ustilago maydis.
  • UmV subtypes P1, P4, and P6 produce distinct killer toxins, affecting sensitive cells within and between species.
  • UmV possesses three dsRNA size classes: H, M, and L, with L segments uniquely originating from M segments.

Purpose of the Study:

  • To sequence and characterize the P1 L dsRNA segment of UmV.
  • To elucidate the origin and replication mechanism of the P1 L dsRNA segment.
  • To understand the unique relationship between M and L dsRNA segments in UmV.

Main Methods:

  • DNA sequencing of the P1 L dsRNA segment.
  • Sequence alignment to determine its relationship with the M segment.

Related Experiment Videos

  • In vitro translation assays to assess protein coding potential.

    • Main Results:

    • The P1 L dsRNA segment was sequenced and mapped to the 3' end of the P1 M1 plus strand.
    • Overlapping regions between P1 L and P1 M1 dsRNAs showed identical sequences.
    • P1 L (355 bp) lacks significant open reading frames and produced no in vitro translation product, while P1 M1 encoded a peptide from its non-L region.

    Conclusions:

    • The P1 L dsRNA segment is derived from the 3' end of the M segment, likely through a novel mechanism.
    • This process involves the replication and packaging of a processed mRNA 3' end fragment.
    • This represents a unique dsRNA virus replication strategy not observed in other known dsRNA viruses.