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A simultaneous two-colour detection method of human IgG- and IgE-reactive proteins from lactic acid bacteria
Lidia Hanna Markiewicz1, Agata Szymkiewicz1, Anna Szyc1
1Department of Immunology and Food Microbiology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima Str. 10, 10-748 Olsztyn, Poland.
Abstract:
Whole cell extracts of two Lactobacillus strains were tested with primary antibodies from two pooled sera from allergic patients. Fluorescently labelled anti-human IgG and anti-human IgE secondary antibodies applied in Western blotting, together with an appropriate image acquisition protocol facilitated imagining bacterial proteins that reacted with human IgG and IgE.
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