Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Peripheral nerve regeneration through optic nerve grafts.

P N Anderson1, P Woodham, M Turmaine

  • 1Department of Anatomy and Developmental Biology, University College, London, Great Britain.

Acta Neuropathologica
|January 1, 1989
PubMed
Summary

Disrupting the glia limitans in optic nerve grafts enhanced regeneration of peripheral nerve fibers. Living astrocytes, not CNS myelin, were identified as the primary barrier to axon extension and Schwann cell migration into central nervous system tissue.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatially resolved common-path high-order harmonic interferometry.

Optics letters·2018
Same author

A comprehensive pathological survey of duodenal biopsies from dogs with diet-responsive chronic enteropathy.

Journal of veterinary internal medicine·2013
Same author

Evaluation of the potential antimicrobial resistance transfer from a multi-drug resistant Escherichia coli to Salmonella in dairy calves.

Current microbiology·2012
Same author

The effect of dietary protein level on performance characteristics of coccidiosis vaccinated and nonvaccinated broilers following mixed-species Eimeria challenge.

Poultry science·2011
Same author

Implantation of the myenteric plexus into the corpus striatum of adult rats: survival of the neurons and glia and interactions with host brain.

Restorative neurology and neuroscience·2011
Same author

Molecular analysis of Salmonella serotypes at different stages of commercial turkey processing.

Poultry science·2010

Area of Science:

  • Neuroscience
  • Regenerative Medicine
  • Ophthalmology

Background:

  • Peripheral nerve injury often results in functional deficits.
  • Central nervous system (CNS) tissue presents a significant barrier to peripheral nerve regeneration.
  • Understanding these barriers is crucial for developing strategies to promote nerve repair.

Purpose of the Study:

  • To investigate factors influencing the penetration of CNS optic nerve grafts by regenerating peripheral axons.
  • To identify the cellular and molecular barriers to axon growth and Schwann cell migration into CNS tissue.
  • To evaluate the potential of optic nerve grafts for bridging peripheral nerve gaps.

Main Methods:

  • Grafting of adult and neonatal optic nerves onto severed common peroneal nerves in mice.

Related Experiment Videos

  • Electron microscopy to examine nerve fiber penetration and cellular composition.
  • Immunohistochemistry using neurofilament antiserum.
  • Horseradish peroxidase (HPP) injection to assess blood-brain barrier permeability.

    • Main Results:

    • Fraying the glia limitans and suturing grafts enhanced axonal penetration.
    • Axonal growth was limited to approximately 1.5 mm within the CNS graft.
    • Schwann cells were rarely found in grafts; degenerating and varicose nerve fibers were common.
    • Living astrocytes, not CNS myelin or lack of trophic factors, were identified as the main barrier to axon extension and Schwann cell migration.

    Conclusions:

    • Modifying the graft interface, such as disrupting the glia limitans, can promote limited regeneration.
    • Astrocytic processes within CNS tissue form a significant barrier to successful peripheral nerve regeneration.
    • Further research is needed to overcome astrocytic barriers for effective nerve repair strategies.