A Flow SPR Immunosensor Based on a Sandwich Direct Method
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Updated: Mar 21, 2026
Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
Published on: November 13, 2017
Mauro Tomassetti1, Giorgia Conta2, Luigi Campanella3
1Department of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy. Mauro.tomassetti@uniroma1.it.
Abstract:
In this study, we report the development of an SPR (Surface Plasmon Resonance) immunosensor for the detection of ampicillin, operating under flow conditions. SPR sensors based on both direct (with the immobilization of the antibody) and competitive (with the immobilization of the antigen) methods did not allow the detection of ampicillin. Therefore, a sandwich-based sensor was developed which showed a good linear response towards ampicillin between 10(-3) and 10(-1) M, a measurement time of ≤20 min and a high selectivity both towards β-lactam antibiotics and antibiotics of different classes.
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