A Flow SPR Immunosensor Based on a Sandwich Direct Method.

Area of Science:

• Analytical Chemistry
• Biosensors
• Immunosensing

Background:

• Antibiotic resistance necessitates rapid and accurate detection methods.
• Surface Plasmon Resonance (SPR) is a label-free detection technology.
• Existing SPR methods for ampicillin detection have limitations.

Purpose of the Study:

• To develop a novel SPR immunosensor for ampicillin detection.
• To overcome limitations of direct and competitive SPR assays.
• To achieve sensitive and selective detection of ampicillin.

Main Methods:

• Development of a sandwich-based SPR immunosensor.
• Immobilization strategies for antibodies and antigens.
• Optimization of assay conditions for ampicillin detection.
• Validation of sensor performance including linearity, response time, and selectivity.

Main Results:

• Direct and competitive SPR methods were unsuccessful for ampicillin detection.
• The developed sandwich-based SPR immunosensor demonstrated a linear response for ampicillin from 10(-3) to 10(-1) M.
• Detection time was less than or equal to 20 minutes.
• The sensor exhibited high selectivity for ampicillin against other β-lactam and different classes of antibiotics.

Conclusions:

• A sandwich-based SPR immunosensor is effective for ampicillin detection.
• The developed sensor offers a rapid, selective, and sensitive method for antibiotic monitoring.
• This technology has potential applications in food safety and clinical diagnostics.