Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

1.1K
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
1.1K
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.7K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.7K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

835
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
835
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

2.5K
NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
2.5K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.8K
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

2.0K
A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prognostic assessment in older adults with solid tumors: A multicenter comparison of Onco-MPI, Balducci's classification, and G8 score.

Journal of geriatric oncology·2026
Same author

Primary adrenal insufficiency in patients with bilateral adrenal metastases treated with curative ablative radiation therapy: A comprehensive review of literature and expert agreements for radiation practice.

Clinical and translational radiation oncology·2026
Same author

Efficacy and safety of adrenalectomy and stereotactic ablative radiotherapy (SABR) for adrenal metastases: A systematic review and meta-analysis.

Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology·2026
Same author

Stereotactic Radiosurgery for Brain Arteriovenous Malformations as Expected Curative Treatment: Outcomes of Patients Included in the Prospective Registry of a Pragmatic Trial.

World neurosurgery·2026
Same author

Hybrid Isoxazole-Hydroxyapatite composites as antimicrobial agents: integrated in silico and in vitro investigation.

Journal of materials science. Materials in medicine·2026
Same author

Life expectancy of people with hemophilia in France in 2022.

European journal of epidemiology·2026

Related Experiment Video

Updated: Mar 21, 2026

Optimizing Magnetic Force Microscopy Resolution and Sensitivity to Visualize Nanoscale Magnetic Domains
07:42

Optimizing Magnetic Force Microscopy Resolution and Sensitivity to Visualize Nanoscale Magnetic Domains

Published on: July 20, 2022

3.5K

Achieving high resolution and optimizing sensitivity in spatial frequency encoding NMR spectroscopy: from theory to

Bertrand Plainchont1, Daisy Pitoux1, Ghanem Hamdoun1

  • 1Equipe de RMN en milieu orienté, ICMMO, UMR 8182 (CNRS-UPS), Université Paris-Saclay, 91405 Orsay cedex, France. nicolas.giraud@u-psud.fr.

Physical Chemistry Chemical Physics : PCCP
|May 19, 2016
PubMed
Summary

This study presents a new model for spatial frequency encoding in Nuclear Magnetic Resonance (NMR) spectroscopy. Optimized NMR techniques offer superior sensitivity and resolution compared to existing methods.

More Related Videos

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.9K
Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST
10:28

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST

Published on: November 2, 2018

12.7K

Related Experiment Videos

Last Updated: Mar 21, 2026

Optimizing Magnetic Force Microscopy Resolution and Sensitivity to Visualize Nanoscale Magnetic Domains
07:42

Optimizing Magnetic Force Microscopy Resolution and Sensitivity to Visualize Nanoscale Magnetic Domains

Published on: July 20, 2022

3.5K
Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.9K
Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST
10:28

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST

Published on: November 2, 2018

12.7K

Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Quantum Chemistry
  • Spectroscopic Techniques

Background:

  • Spatial frequency encoding is crucial for advanced NMR experiments.
  • Understanding gradient-encoded pulses is key to optimizing NMR signal localization.
  • Previous methods lacked detailed theoretical models for spatial properties.

Purpose of the Study:

  • To develop a theoretical model for simulating gradient-encoded pulses in NMR.
  • To analyze the spatial properties of locally created NMR signals.
  • To investigate factors influencing slice selection efficiency in excitation and refocusing pulses.

Main Methods:

  • Development of a theoretical model for gradient-encoded pulses.
  • Simulation of spatial properties of NMR signals.
  • Experimental investigation on a model ABX spin system.
  • Analysis of pure shift and J-edited NMR experiments.

Main Results:

  • A theoretical model accurately describes spatial properties of NMR signals.
  • Key features affecting slice selection efficiency were identified.
  • Optimized spatial frequency encoding enhances sensitivity and resolution.
  • Pure shift and J-edited experiments show improved performance.

Conclusions:

  • The developed theoretical model provides a robust framework for spatial frequency encoding in NMR.
  • Optimized NMR techniques based on spatial frequency encoding offer superior performance.
  • This approach enables enhanced high-resolution NMR spectroscopy.