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Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay
Published on: September 6, 2016
ELISA and Neutralization Methods to Measure Anti-West Nile Virus Antibody Responses
Brian M Friedrich1, David W C Beasley2
1Department of Microbiology and Immunology, Sealy Center for Vaccine Development, Institute for Human Infections and Immunity, University of Texas Medical Branch, MRB, Route 0609, 301 University Blvd., Galveston, TX, USA.
Abstract:
Measurements of humoral immune responses to West Nile virus (WNV) infection in mouse or other animal models are valuable components of basic laboratory investigations to assess immunogenicity of candidate vaccines or to evaluate seroconversion following challenge with WNV. Here, we outline the steps for screening or titrating of total antibodies by indirect enzyme linked immunosorbent assay (ELISA) as well as assessment of neutralizing antibody titers by immunofocus detection.

