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Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay
Published on: June 9, 2011
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Field Surveillance Methods for West Nile Virus.
Samuel B Jameson1, Dawn M Wesson2
1Department of Tropical Medicine, School of Public Health and Tropical Medicine, Tulane University, JBJ Building, Room 509, 1430 Tulane Ave., SL-17, New Orleans, LA, 70119, USA.
Methods in Molecular Biology (Clifton, N.J.)
|May 19, 2016
Summary
Monitoring West Nile virus (WNV) in mosquitoes and animals is crucial for assessing human health risks. This chapter details methods for detecting WNV and its transmission, aiding public health surveillance efforts.
Area of Science:
- Veterinary Entomology
- Arbovirology
- Public Health Entomology
Background:
- West Nile virus (WNV) poses a significant threat to human health through natural transmission cycles.
- Effective surveillance is essential for understanding and mitigating WNV risks.
Purpose of the Study:
- To outline key methods for detecting West Nile virus (WNV).
- To describe techniques for identifying WNV enzootic transmission in animal populations.
Main Methods:
- Virus detection methods: CDC mini light trap, CDC gravid trap, and dead bird surveillance.
- Transmission detection methods: passive box traps, chicken-baited traps, and sentinel chickens.
Main Results:
- The chapter provides a comprehensive overview of established WNV surveillance techniques.
- It details practical approaches for both direct virus detection and indirect transmission monitoring.
Conclusions:
- Accurate surveillance of WNV in mosquito and animal populations is vital for public health protection.
- The described methods offer valuable tools for researchers and public health officials managing WNV.

