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Detection Protocols for West Nile Virus in Mosquitoes, Birds, and Nonhuman Mammals

Elizabeth B Kauffman1, Mary A Franke2, Laura D Kramer2,3

  • 1Wadsworth Center, New York State Department of Health, 5668 State Farm Road, Slingerlands, NY, 12159, USA. elizabeth.kauffman@health.ny.gov.

Methods in Molecular Biology (Clifton, N.J.)
|May 19, 2016
PubMed

Keywords:
ELISAFlavivirusPRNTRT-PCRSurveillanceWest Nile

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Arboviral encephalitis refers to brain inflammation caused by arthropod-borne viruses, particularly those transmitted through mosquito vectors. Among these, West Nile virus (WNV), a member of the Flaviviridae family, is a significant public health concern. WNV is an enveloped, positive-sense, single-stranded RNA virus. Human infection typically begins when an infected mosquito introduces the virus into the dermis during feeding. The primary transmission cycle involves birds as amplifying hosts...
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