Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Indoor thermoregulatory homeostasis using hydrodynamic instability.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Rotational 3D printing of active-passive filaments and lattices with programmable shape morphing.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Liquid-infused silicone catheters reduce fungal burden and inflammation in <i>Candidozyma auris</i> bladder infections.

mSphere·2026
Same author

Self-regulated dual-mode solar energy harvesting.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Liquid-Infused Silicone Catheters Reduce Fungal Burden and Inflammation in <i>Candidozyma auris</i> Bladder Infections.

bioRxiv : the preprint server for biology·2026
Same author

Topography of the HLA-A protein enforces shared and convergent immunodominant B cell and antibody alloresponses in transplant recipients.

Immunity·2025
Same journal

MT-MRI for detection of renal interstitial fibrosis in renovascular disease.

Scientific reports·2026
Same journal

Detection of underground objects from GPR data using a lightweight YOLO-based approach.

Scientific reports·2026
Same journal

Early systemic inflammatory-metabolic trajectory phenotypes are associated with survival outcomes in metastatic renal cell carcinoma treated with nivolumab.

Scientific reports·2026
Same journal

Water balance components in a dry-seeded rice-wheat system: Untangling the effects of tillage and mulching practices.

Scientific reports·2026
Same journal

Topological approaches to quantum tensor train compression via ZX-calculus and SVD.

Scientific reports·2026
Same journal

determinants of flood impacts and adaptive capacity among market vendors in Walukuba-Masese, Jinja city, Uganda.

Scientific reports·2026
See all related articles

Related Experiment Video

Updated: Mar 21, 2026

Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules
11:13

Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules

Published on: August 19, 2015

8.8K

Infused polymers for cell sheet release.

Nidhi Juthani1,2, Caitlin Howell1,2,3, Haylea Ledoux1

  • 1Wyss Institute for Biologically Inspired Engineering, 60 Oxford Street, Cambridge, Massachusetts 02138, United States.

Scientific Reports
|May 19, 2016
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel, low-cost cell sheet release surface using silicone oil-infused polydimethylsiloxane. This method enables easy detachment of intact cell sheets for tissue engineering applications, maintaining cell viability and function.

More Related Videos

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering
12:22

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

Published on: March 1, 2016

8.8K
Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications
09:39

Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications

Published on: February 7, 2021

9.1K

Related Experiment Videos

Last Updated: Mar 21, 2026

Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules
11:13

Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules

Published on: August 19, 2015

8.8K
Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering
12:22

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

Published on: March 1, 2016

8.8K
Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications
09:39

Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications

Published on: February 7, 2021

9.1K

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Cell Biology

Background:

  • Cell sheet technology is promising for tissue engineering but faces challenges with expensive and complex fabrication of release systems.
  • Current methods for cell sheet harvesting can be costly and difficult to implement, limiting broader application in cell-based therapies.

Purpose of the Study:

  • To develop a new, cost-effective, and user-friendly surface for the growth and detachment of intact cell sheets for tissue engineering.
  • To investigate the efficacy of silicone oil-infused polydimethylsiloxane coated with fibronectin as a cell sheet release substrate.

Main Methods:

  • Fabrication of silicone oil-infused polydimethylsiloxane surfaces.
  • Coating surfaces with fibronectin (FN).
  • Culture of mesenchymal stem cells (MSCs) on coated surfaces and assessment of cell density compared to tissue culture polystyrene (TCPS).
  • Detachment of cell sheets using oil introduction.
  • Post-transfer characterization of cell sheet morphology, viability, and proliferative capacity.

Main Results:

  • Mesenchymal stem cells (MSCs) achieved densities comparable to tissue culture polystyrene controls (TCPS) on the novel surfaces.
  • Simple introduction of oil facilitated easy detachment of intact cell sheets.
  • Transferred cell sheets maintained their fibronectin (FN) layer, morphology, and high viability.
  • Cells demonstrated normal growth and proliferation post-transfer.

Conclusions:

  • Silicone oil-infused polydimethylsiloxane surfaces offer a low-cost, flexible, and customizable method for cell sheet production.
  • This approach facilitates efficient detachment of viable cell sheets for tissue engineering.
  • The developed method has the potential to advance the widespread use of cell sheet-based therapies.