Related Experiment Video
Updated: Mar 21, 2026
08:32
Conducting Multiple Imaging Modes with One Fluorescence Microscope
Published on: October 28, 2018
10.4K
Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets
Teng Zhao1, Sze Cheung Lau1, Ying Wang1
1Department of Physics, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
Scientific Reports
|May 19, 2016
Summary
Researchers developed a cost-effective method to create ultrathin Bessel light sheets in any color. This technique enables high-resolution 3D and 4D imaging of biological samples, advancing microscopy capabilities.
Area of Science:
- Optics and Photonics
- Biomedical Imaging
- Microscopy
Background:
- Bessel light sheets offer advantages for 3D microscopy due to their non-diffracting nature.
- Existing methods for generating Bessel light sheets can be complex or limited in color flexibility.
Purpose of the Study:
- To present a simple, efficient, and cost-effective method for producing ultrathin Bessel light sheets of any color.
- To demonstrate the application of this technology for high-resolution 3D and 4D biological imaging.
Main Methods:
- Utilized a line-shaped beam in conjunction with an annulus filter to generate ultrathin Bessel light sheets.
- Employed a robust and cost-effective optical setup for light sheet generation.
Main Results:
- Achieved production of ultrathin Bessel light sheets across a spectrum of colors.
- Obtained two-color, 3D images of biological samples with 250 nm lateral and 400 nm axial resolution.
- Performed high-speed, 4D volume imaging of live samples (20 μm) at 1 Hz temporal resolution.
Conclusions:
- The developed method provides a versatile and accessible tool for advanced light sheet microscopy.
- This technology facilitates high-resolution and high-speed imaging of biological structures and dynamics.
Related Concept Videos
01:37Super-resolution Fluorescence Microscopy
14.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.8K
01:16Confocal Fluorescence Microscopy
21.8K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.8K
01:28Three-Dimensional Microscopy in Microbiology
970
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
970