Related Experiment Video
Updated: Mar 21, 2026
A Platform of Anti-biofilm Assays Suited to the Exploration of Natural Compound Libraries
Published on: December 27, 2016
Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics - An In-vitro
Amulya Vanapatla1, Harikumar Vemisetty2, Rajani Punna1
1Senior Lecturer, Department of Conservative Dentistry and Endodontics, Kamineni Institute of Dental Sciences , Narketpally, Nalgonda, Telangana, India .
Introduction:
Root canal sealers with good sealing ability and antimicrobial activity are desired to entomb the surviving microorganisms.
Aim:
Aim of the present study is to evaluate the antimicrobial effect of different sealers mixed with antibiotics.
Materials And Methods:
Sixty extracted premolars were taken and were cut coronally & apically such that 7mm of tooth specimen was prepared. They were sterilized by autoclaving, inoculated with E.faecalis and incubated for three weeks. The specimens were divided into six groups of 10 each. Group 1 - ZnOE, Group 2 - ZnOE +TAM, Group 3 - Apexit Plus, Group 4 - Apexit Plus +TAM, Group 5 - AH Plus, Group 6 - AH Plus + TAM. Bacterial growth in the each specimen was calculated before & after sealer application and noted as initial & final colony count. Antimicrobial effect of each sealer was measured by calculating the Percentage Reduction in Colony Count (%RCC). One way analysis of variance and post hoc tests were used for statistical analysis.
Results:
ZnOE + TAM group showed maximum antibacterial effect among the sealers tested and AH Plus sealer showed least antimicrobial effect.
Conclusion:
When the sealers were mixed with triple antibiotic mixture the antimicrobial effect was increased significantly.
More Related Videos
08:20Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries
Published on: March 31, 2021
11:52Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
Published on: April 21, 2023
