Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Voltage-driven, taurocholate-dependent secretion in isolated hepatocyte couplets.

S A Weinman1, J Graf, J L Boyer

  • 1Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06510.

The American Journal of Physiology
|May 1, 1989
PubMed
Summary

Liver bile secretion is driven by membrane voltage. Altering hepatocyte membrane potential significantly impacts bile acid transport and fluid secretion, supporting an electrodiffusion mechanism for bile formation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploring 'decision regret' in adjuvant-treated melanoma patients: Results from a cross-sectional survey on 200 participants.

Journal of the European Academy of Dermatology and Venereology : JEADV·2024
Same author

Investigation of liver-targeted peripheral focused ultrasound stimulation (pFUS) and its effect on glucose homeostasis and insulin resistance in type 2 diabetes mellitus: a proof of concept, phase 1 trial.

QJM : monthly journal of the Association of Physicians·2023
Same author

Activity tracker-based intervention to increase physical activity in patients with type 2 diabetes and healthy individuals: study protocol for a randomized controlled trial.

Trials·2022
Same author

Addressing barriers to mental health services: evaluation of a psychoeducational short film for forcibly displaced people.

European journal of psychotraumatology·2022
Same author

High-Resolution Differentiation of Enteric Bacteria in Premature Infant Fecal Microbiomes Using a Novel rRNA Amplicon.

mBio·2021
Same author

Citizen science in hydrological monitoring and ecosystem services management: State of the art and future prospects.

The Science of the total environment·2019

Area of Science:

  • Hepatology
  • Cell Physiology
  • Membrane Transport

Background:

  • Bile formation relies on hepatocyte bile acid transport.
  • This process involves Na-coupled uptake and voltage-dependent canalicular transport.
  • The direct influence of membrane potential on bile secretion remains uncharacterized.

Purpose of the Study:

  • To investigate the effect of altered membrane potential on bile secretion.
  • To determine if membrane voltage acts as a driving force for bile acid transport.

Main Methods:

  • Rat hepatocyte couplets were impaled with microelectrodes.
  • Intracellular current injection was used to manipulate membrane potential.
  • Canalicular size was optically measured to quantify fluid secretion.

Related Experiment Videos

Main Results:

  • A hyperpolarized membrane potential (-109.9 mV) increased canalicular area by 20%/min (19 fl/min) in the presence of taurocholate.
  • A less negative potential (-33.3 mV) resulted in a 6%/min increase (2-4 fl/min).
  • Hyperpolarization had no effect on canalicular size without taurocholate, indicating taurocholate dependence.

Conclusions:

  • Canalicular bile secretion is dependent on membrane voltage.
  • Electrodiffusion is a likely mechanism for taurocholate-dependent bile secretion.
  • Membrane voltage is a key driving force for liver fluid secretion.