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Updated: Mar 21, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
Published on: May 30, 2016
Enhanced super resolution using Fresnel incoherent correlation holography with structured illumination
Abstract:
The structured illumination (SI) technique has already been well established as a resolution enhancer in many studies and well demonstrated in many optical imaging systems during the past decade. The ability to use the SI in incoherent imaging systems was also introduced, especially in fluorescence microscopy. In this Letter, we propose and demonstrate a new approach to combine the SI technique with the recently innovated motionless incoherent holographic system, called Fresnel incoherent correlation holography (FINCH), in order to enhance the resolution beyond the limits achieved in regular imaging with SI. The results obtained by use of SI-FINCH were compared against regular imaging, regular FINCH and SI-imaging.
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