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Change in the selection of microRNA strands during DNA damage induction

V A Tarasov1, M A Makhotkin2, E F Shin1

  • 1Institute of Arid Zones, Southern Scientific Center, Russian Academy of Sciences, pr. Chekhova 41, Rostov-on-Don, 3444006, Russia.

Doklady. Biochemistry and Biophysics
|May 20, 2016
PubMed

Insights

DNA damage affects microRNA strand selection during RNA-induced silencing complex (RISC) formation. Mitomycin C treatment in HeLa cells alters how 5p and 3p microRNA duplexes are incorporated into RISC.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are key regulators of gene expression.
  • The RNA-induced silencing complex (RISC) mediates miRNA function.
  • Strand selection of miRNA duplexes is crucial for RISC activity.

Purpose of the Study:

  • To investigate the impact of DNA damage on miRNA duplex strand selection.
  • To determine if DNA damage affects the incorporation of 5p and 3p miRNA strands into RISC.

Main Methods:

  • HeLa cells were treated with mitomycin C to induce DNA damage.
  • Analysis of miRNA duplex strand selection during RISC assembly.
  • Quantitative assessment of 5p and 3p miRNA incorporation.

Main Results:

  • DNA damage induction significantly altered the selection of miRNA duplex strands.
  • Mitomycin C treatment led to a measurable change in the ratio of 5p and 3p miRNA strands incorporated into RISC.
  • This suggests DNA damage influences the loading process of miRNAs into the RISC.

Conclusions:

  • DNA damage is a critical factor influencing miRNA strand selection in RISC.
  • The findings reveal a novel mechanism by which DNA damage can modulate gene silencing pathways.
  • This has implications for understanding cellular responses to genotoxic stress.

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