Related Experiment Video
Updated: Mar 21, 2026

Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles
Published on: November 15, 2019
Imaging and Quantifying the Dynamics of γ-Tubulin at Microtubule Minus Ends in Mitotic Spindles
Nicolas Lecland1, Jens Lüders2
1Centre Biologie du Développement, UMR 5547 CNRS-Université Paul Sabatier, Toulouse, France.
Abstract:
Understanding the organization of complex microtubule arrays such as the mitotic spindle requires information about the position and dynamics of microtubule plus and minus ends. Whereas plus end dynamics have been widely studied using markers such as EB1-GFP, much less is known about the dynamic properties of minus ends, in part because a suitable marker has only recently become available. Here we describe the use of photoactivatable γ-tubulin-paGFP to image and quantify the dynamics of microtubule minus ends in mitotic spindles.
More Related Videos
Related Concept Videos
Anaphase A and B
Plus-end depolymerization releases tubulin heterodimers from the terminal region of the microtubule. As tubulin subunits are lost, the Ndc80 complexes detach...
Microtubule Instability
Destabilization of Microtubules
The Mitotic Spindle
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures...
Microtubule Formation
Forces Acting on Chromosomes
Microtubules and motor proteins exert two types of forces on...

