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Updated: Mar 21, 2026

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
Published on: September 20, 2019
Analyzing Spindle Positioning Dynamics in Cultured Cells
1Division of Biological Science, Graduate School of Science, Nagoya University, Room 227 Building A, Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan. kiyomitsu@bio.nagoya-u.ac.jp.
Abstract:
As spindle positioning during mitosis is a highly dynamic process, live cell imaging is a key technology when studying its underlying mechanisms. Recent advances in imaging tools and microscope systems have enabled us to simultaneously visualize several cellular components in living cells with high temporal and spatial resolution. By combining live cell imaging with functional assays such as RNAi-based depletions, drug inhibition, and micropatterned coverslips, novel and unexpected mechanisms of spindle positioning have been uncovered. In this chapter, I present methods for analyzing the dynamics of spindle positioning in cultured cells.
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