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Mitosis and Cytokinesis

Mitosis and Cytokinesis

In eukaryotes, the cell division cycle is divided into distinct, coordinated cellular processes that include cell growth, DNA replication/chromosome duplication, chromosome distribution to daughter cells, and finally, cell division. The cell cycle is tightly regulated by its regulatory systems as well as extracellular signals that affect cell proliferation.
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...

Mitosis and Cytokinesis

Mitosis and Cytokinesis

In eukaryotes, the cell division cycle is divided into distinct, coordinated cellular processes that include cell growth, DNA replication/chromosome duplication, chromosome distribution to daughter cells, and finally, cell division. The cell cycle is tightly regulated by its regulatory systems as well as extracellular signals that affect cell proliferation.
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...

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Live Imaging Characterization of Centromere Movements During Male Meiotic Prophase in Arabidopsis thaliana

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Imaging Mitosis in the Moss Physcomitrella patens.

Moé Yamada¹, Tomohiro Miki¹, Gohta Goshima²

¹Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan.

Methods in Molecular Biology (Clifton, N.J.)

|May 20, 2016

Summary

Plant mitosis, unlike animal mitosis, lacks centrosomes and utilizes a unique phragmoplast. This study introduces advanced imaging techniques in Physcomitrella patens for high-throughput analysis of plant cell division.

Keywords:

Caulonemal apical cell GFP tagging Kinesin Mitosis Physcomitrella patens

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Last Updated: Mar 21, 2026

Live Imaging Characterization of Centromere Movements During Male Meiotic Prophase in Arabidopsis thaliana

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Published on: October 24, 2025

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Published on: June 4, 2014

Live Cell Imaging of Chromosome Segregation During Mitosis

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

Area of Science:

Cell Biology
Molecular Biology
Developmental Biology

Background:

Plant mitosis differs significantly from animal mitosis, notably lacking centrosomes.
The plant-specific phragmoplast is a key structure in cell division after anaphase.
Understanding plant mitotic protein repertoires is challenging due to difficulties in gene identification and characterization.

Purpose of the Study:

To present protocols for advanced mitosis imaging in the model moss, Physcomitrella patens.
To enable high-throughput studies of plant cell division and intracellular processes.
To facilitate the in vivo characterization of plant mitotic genes.

Main Methods:

Utilizing endogenous green fluorescent protein (GFP) tagging for protein localization.
Employing conditional RNA interference (RNAi) for gene function analysis.
Applying advanced microscopy techniques for mitosis imaging in Physcomitrella patens.

Main Results:

Established protocols for combining GFP tagging and RNAi with mitosis imaging.
Demonstrated the potential of Physcomitrella patens as a model for high-throughput cell biology studies.
Provided a framework for investigating plant-specific mitotic mechanisms.

Conclusions:

Physcomitrella patens offers a powerful system for studying plant mitosis.
The developed protocols can accelerate the discovery of new mitotic regulators in plants.
This approach bridges the gap in understanding plant versus animal cell division at the molecular level.