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Related Concept Videos

Spindle Assembly02:50

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Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
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The Mitotic Spindle02:27

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The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
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Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...
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The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
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Related Experiment Video

Updated: Mar 21, 2026

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs
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Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

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Identification and Characterization of Mitotic Spindle-Localized Transcripts.

Amy B Emerman1,2, Ashwini Jambhekar1,2, Michael D Blower3,4

  • 1Department of Molecular Biology, Massachusetts General Hospital, Boston, MA, 02114, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 20, 2016
PubMed
Summary

Researchers developed a method to identify RNAs on the mitotic spindle using Xenopus egg extracts. This technique helps understand RNA

Keywords:
Mitotic SpindleRNA localizationRNaseAXenopus laevis

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • RNAs are found associated with the mitotic spindle in diverse organisms.
  • Spindle-associated RNAs play roles in protein production, chromosome segregation, and spindle assembly.
  • Understanding these RNAs is crucial for deciphering their biological functions.

Purpose of the Study:

  • To describe a method for isolating mitotic spindles and identifying associated RNAs.
  • To enable investigation of factors influencing RNA localization to the spindle.
  • To provide a means to study the impact of RNA depletion on spindle formation.

Main Methods:

  • Utilizing Xenopus laevis egg extracts to assemble and isolate mitotic spindles.
  • Employing techniques like immunodepletions and inhibitor additions to perturb RNA-spindle interactions.
  • Developing a method to assess the effects of RNA ablation on spindle formation.

Main Results:

  • Successfully established a protocol for spindle assembly and RNA isolation from Xenopus egg extracts.
  • Demonstrated the utility of the method for investigating RNA-spindle dynamics under various conditions.
  • Provided a framework for functional studies on spindle-associated RNAs.

Conclusions:

  • The described method is effective for identifying spindle-associated RNAs in Xenopus egg extracts.
  • This approach facilitates the study of RNA localization and function during mitosis.
  • Further research can elucidate the precise roles of these RNAs in cell division.