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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Updated: Mar 21, 2026

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer
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Economic methods used in fabrication of tissue microarray: A pilot study.

Sahana Srinath1, Rohit Kumar Kendole1, Pavana Gopinath1

  • 1Department of Oral and Maxillofacial Pathology, Government Dental College and Research Institute, Bengaluru, Karnataka, India.

Journal of Oral and Maxillofacial Pathology : JOMFP
|May 20, 2016
PubMed
Summary

This study presents an inexpensive and efficient method for fabricating tissue microarray (TMA) molds using departmental silicone. This novel approach offers advantages over existing expensive automated tissue arrayers and commercial molds.

Keywords:
Multiple small tubular structuressilicone moldstissue microarray

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Area of Science:

  • Biotechnology
  • Histopathology
  • Medical Diagnostics

Background:

  • Tissue microarray (TMA) enables high-throughput analysis of numerous tissue samples simultaneously.
  • Conventional TMA construction methods using automated arrayers or commercial molds are costly.
  • There is a need for cost-effective alternatives for TMA fabrication.

Purpose of the Study:

  • To develop and evaluate an inexpensive method for fabricating tissue microarray (TMA) molds.
  • To compare the efficacy of a custom-made silicone mold with existing TMA construction techniques.

Main Methods:

  • A silicone-based additional silicone mold was fabricated in-house.
  • Tissue cores were prepared from donor blocks and inserted into recipient blocks.
  • The custom silicone mold method was compared against the double-sided adhesive tape technique and a punching hole method.

Main Results:

  • The tissue microarray (TMA) fabricated using the in-house silicone mold demonstrated superior advantages compared to the other two methods.
  • The silicone mold method proved to be more efficient for TMA construction.

Conclusions:

  • Fabricating tissue microarray (TMA) molds using silicone in a departmental setting is a cost-effective solution.
  • The developed silicone mold method is both inexpensive and efficient for TMA construction, offering a viable alternative for research and diagnostics.