Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inorganic Nitrogen Assimilation01:22

Inorganic Nitrogen Assimilation

766
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
766
Overview of Nitrogen Metabolism01:20

Overview of Nitrogen Metabolism

12.5K
Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of  ammonia, ammonium ions, nitrate, nitrite, or  nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
12.5K
Carbon-dioxide Fixation01:28

Carbon-dioxide Fixation

845
Carbon dioxide fixation in prokaryotes enables the assimilation of inorganic carbon into organic molecules, supporting biosynthetic pathways, sustaining ecosystems, and contributing to the global carbon cycle. It also has industrial applications in carbon capture and bioproduct synthesis. Autotrophic organisms rely on this process to utilize CO₂ as a carbon source in diverse environments.The Calvin CycleThe Calvin cycle is the most widespread carbon fixation mechanism, primarily used by...
845
The Nitrogen Cycle01:49

The Nitrogen Cycle

61.3K
Nitrogen atoms, present in all proteins and DNA, are recycled between abiotic and biotic components of the ecosystem. However, the primary form of nitrogen on Earth is nitrogen gas, which cannot be used by most animals and plants. Thus, nitrogen gas must first be converted into a usable form by nitrogen-fixing bacteria before it can be cycled through other living organisms. The use of nitrogen-containing fertilizers and animal waste products in human agriculture has greatly influenced the...
61.3K
The Roles of Bacteria and Fungi in Plant Nutrition02:11

The Roles of Bacteria and Fungi in Plant Nutrition

48.3K
Plants have the impressive ability to create their own food through photosynthesis. However, plants often require assistance from organisms in the soil to acquire the nutrients they need to function correctly. Both bacteria and fungi have evolved symbiotic relationships with plants that help the species to thrive in a wide variety of environments.
48.3K
Overview of Metabolism01:40

Overview of Metabolism

40.8K
Living cells constantly carry out various chemical reactions which are necessary for their proper functioning. These reactions are interlinked to one another via multiple pathways. The collection of these chemical reactions is known as metabolism.
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
40.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neuro-Ocular Amyloid Characterization in Alzheimer's Disease via Cross-Site PET-MRI and Hierarchical Cross-Attention Driven Multimodal Representation Learning.

NeuroImage·2026
Same author

Long-read deep sequencing reveals high rates of multilineage transmission and rapid viral population changes in acute HIV infection.

Nature communications·2026
Same author

Endophyte-induced systemic spatial reprogramming of metabolism in Populus trichocarpa roots under drought.

The New phytologist·2026
Same author

Influence of the broadly neutralizing antibody VRC01 on HIV breakthrough virus populations in antibody-mediated prevention trials.

Nature communications·2026
Same author

AttCo: Attention-based co-Learning fusion of deep feature representation for medical image segmentation using multimodality.

Neural networks : the official journal of the International Neural Network Society·2026
Same author

Sphingobium salicis sp. nov., a novel endophyte with two subspecies from pioneer plants growing on primary substrates.

Antonie van Leeuwenhoek·2025
Same journal

Epidemiological characteristics of amebiasis in Japan from 2001 to 2022.

PloS one·2026
Same journal

Longitudinal associations of academic stress with eating related patterns, nutrition, somatic indicators, and depressive symptoms in university students: A study protocol.

PloS one·2026
Same journal

Pollution removal efficiency enhancement by agricultural biomass additions in constructed wetlands: A framework integrating meta-analysis with explainable machine learning.

PloS one·2026
Same journal

Insulation failure mapping on power transformer bushing using FRA and electrostatic simulation.

PloS one·2026
Same journal

Enhancing medical Q&A systems with multimodal knowledge graphs and dual-layer attention mechanisms.

PloS one·2026
Same journal

UAMP: Consistent video object segmentation with uncertainty-aware memory propagation.

PloS one·2026
See all related articles

Related Experiment Video

Updated: Mar 21, 2026

Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes
08:04

Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes

Published on: October 11, 2024

561

Variable Nitrogen Fixation in Wild Populus.

Sharon L Doty1, Andrew W Sher1, Neil D Fleck1

  • 1School of Environmental and Forest Sciences, College of the Environment, University of Washington, Seattle, Washington, United States of America.

Plos One
|May 20, 2016
PubMed
Summary
This summary is machine-generated.

Wild poplar trees (Populus trichocarpa) can fix nitrogen (N2) through endophytic microorganisms, a process previously unconfirmed in non-leguminous trees. This discovery highlights potential microbial contributions to plant nutrition in low-N environments.

More Related Videos

Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii
12:22

Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii

Published on: August 18, 2019

13.9K
Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana
07:33

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

Published on: January 3, 2014

21.4K

Related Experiment Videos

Last Updated: Mar 21, 2026

Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes
08:04

Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes

Published on: October 11, 2024

561
Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii
12:22

Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii

Published on: August 18, 2019

13.9K
Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana
07:33

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

Published on: January 3, 2014

21.4K

Area of Science:

  • Plant Microbiology
  • Nitrogen Fixation
  • Symbiotic Relationships

Background:

  • Plant microbiomes are crucial for health and nutrient acquisition.
  • Legumes and actinorhizal plants utilize symbiotic nitrogen fixation.
  • Non-nodulating plants may use endophytes for nitrogen acquisition in low-N soils.

Purpose of the Study:

  • To provide the first direct evidence of nitrogen (N2) fixation in Populus trichocarpa, an early successional wild tree.
  • To investigate the role of endophytic microorganisms in nitrogen acquisition for non-leguminous trees.

Main Methods:

  • Assaying surface-sterilized Populus trichocarpa cuttings using 15N2 incorporation.
  • Employing the acetylene reduction assay to measure N2 fixation activity.
  • Analyzing the bacterial microbiota diversity and numbers within cuttings.

Main Results:

  • High levels of 15N label incorporation were observed in a subset of cuttings.
  • Acetylene reduction to ethylene was detected in some samples, indicating N2 fixation.
  • Significant variability in bacterial numbers and genetic diversity was found among cuttings.

Conclusions:

  • Associative N2 fixation occurs within wild poplar (Populus trichocarpa).
  • Variability in endophytic bacterial distribution may explain differences in N-fixation activity.
  • Further molecular studies are needed to identify microbial consortia for effective endophytic N2 fixation in trees.