Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Stringent Response in E. coli01:23

Stringent Response in E. coli

462
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
462
Genomic DNA in Prokaryotes00:46

Genomic DNA in Prokaryotes

50.3K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
50.3K
Microbiota of the Urogenital Tract01:28

Microbiota of the Urogenital Tract

11
The human urogenital system, once thought to be sterile in healthy individuals, is now recognized as a complex microbial habitat. Advancements in molecular sequencing techniques have revealed that even in healthy adults, the kidneys and bladder harbor microbial populations similar to those found in the distal urethra, albeit in much lower abundance. These resident microorganisms, while generally innocuous, can become opportunistic pathogens under conditions that alter the urogenital...
11
Genomic DNA in Eukaryotes00:58

Genomic DNA in Eukaryotes

53.9K
Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
53.9K
Bacterial Gastroenteritis01:18

Bacterial Gastroenteritis

10
Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid...
10
Bacterial Phylum Firmicutes01:27

Bacterial Phylum Firmicutes

1.2K
Firmicutes is a diverse phylum of Gram-positive bacteria characterized by a low GC content in their genomes. This phylum includes organisms with monoderm or diderm cell envelopes, highlighting a complex evolutionary history. Firmicutes comprises several major orders, including Lactobacillales, Clostridiales, and Bacillales, which exhibit remarkable diversity in their morphology, metabolism, and ecological roles.The order Lactobacillales includes lactic acid bacteria, which are fermentative...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microbiome histidine competition mediates dietary control of systemic imidazole propionate.

bioRxiv : the preprint server for biology·2026
Same author

Host metabolism can produce many indoles and phenols independently of the microbiome.

Nature metabolism·2026
Same author

Biofilm dispersion in <i>Enterococcus faecalis</i> is mediated by nutrient step-change and intra-species signaling.

bioRxiv : the preprint server for biology·2026
Same author

Intracellular acidification by microbiota-derived valeric acid facilitates trans-kingdom ecology limiting Candida parapsilosis colonization.

Cell host & microbe·2026
Same author

Unravelling ring chromosome structures and formation mechanisms by short-read and long-read genomic sequencing.

Genetics in medicine open·2026
Same author

Β-Resorcylaldehyde attenuates DSS-induced colitis by inhibiting inflammation and oxidative stress through the Keap1/Nrf2/HO-1 pathway.

International immunopharmacology·2026

Related Experiment Video

Updated: Mar 21, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

3.0K

Complete Genome Sequence of Enterococcus faecium ATCC 700221.

Peter T McKenney1, Lilan Ling2, Guilin Wang3

  • 1Immunology Program, Infectious Diseases Service, Department of Medicine, Lucille Castori Center for Microbes, Inflammation, and Cancer, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York City, New York, USA mckennep@mskcc.org pamere@mskcc.org.

Genome Announcements
|May 21, 2016
PubMed
Summary

We sequenced the genome of vancomycin-resistant Enterococcus faecium from human feces. This common gut bacterium harbors the vanA gene on a plasmid, contributing to its resistance.

More Related Videos

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
12:14

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

Published on: March 9, 2016

10.4K
A 1.5 Hour Procedure for Identification of Enterococcus Species Directly from Blood Cultures
05:02

A 1.5 Hour Procedure for Identification of Enterococcus Species Directly from Blood Cultures

Published on: February 10, 2011

25.2K

Related Experiment Videos

Last Updated: Mar 21, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

3.0K
A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
12:14

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

Published on: March 9, 2016

10.4K
A 1.5 Hour Procedure for Identification of Enterococcus Species Directly from Blood Cultures
05:02

A 1.5 Hour Procedure for Identification of Enterococcus Species Directly from Blood Cultures

Published on: February 10, 2011

25.2K

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Enterococcus faecium is a significant opportunistic pathogen.
  • Vancomycin resistance in E. faecium is a growing public health concern.
  • Understanding the genetic basis of resistance is crucial for treatment strategies.

Purpose of the Study:

  • To perform a complete genome sequencing of a vancomycin-resistant Enterococcus faecium isolate.
  • To identify the genetic elements, particularly resistance genes and their location, within the sequenced genome.
  • To characterize the isolate's genetic lineage using Multilocus Sequence Typing (MLST).

Main Methods:

  • Whole-genome sequencing of the Enterococcus faecium isolate.
  • Bioinformatic analysis to assemble the genome and identify genes.
  • Plasmid analysis to determine the location of the vancomycin resistance locus.
  • Multilocus Sequence Typing (MLST) for strain characterization.

Main Results:

  • The complete genome sequence of the vancomycin-resistant Enterococcus faecium isolate was obtained.
  • The genome consists of a 2.9 Mb chromosome and three plasmids.
  • The vanA gene, conferring vancomycin resistance, was identified on a plasmid.
  • The isolate belongs to MLST cluster ST-17.

Conclusions:

  • The vanA-type vancomycin resistance in this E. faecium isolate is plasmid-mediated.
  • The genomic data provides insights into the genetic makeup of a clinically relevant resistant strain.
  • Identification of MLST ST-17 highlights the prevalence of this lineage among resistant E. faecium.